外周血miR-30表达水平与气道炎症指标以及哮喘严重程度的相关性分析

目的探讨患者外周血miR-30表达与气道炎症指标以及哮喘严重程度的相关性分析。方法选择2021年1月至2023年6月本院收治的哮喘急性发作患者80例为观察组以及同期健康体检人群80例为对照组。观察组根据病情严重程度分为轻度组(n=28)、中度组(n=34)、重度组(n=18)。比较对照组与观察组外周血miR-30水平、气道炎症指标[转化生长因子-β1(TGF-β1)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-33(IL-33)],分析其与患者哮喘病情程度的关系。结果观察组与对照组miR-30、TGF-β1、IL-6、IL-33、TNF-α比较,差异有统计学意义(P<0.05);轻度组、中度组、重度组三组miR-30、TGF-β1、IL-6、IL-33、TNF-α、嗜酸性粒细胞(EOS)、呼出气一氧化氮(FeNO)水平比较,差异有统计学意义(P<0.05);相关性分析外周血miR-30与TGF-β1、IL-6、IL-33、TNF-α水平呈正相关(r>0,P<0.05);Logistic回归分析外周血miR-30、TGF-β1、TNF-α、FeNO、EOS是哮喘病情程度的影响因素(P<0.05)。结论外周血miR-30在哮喘患者中异常高表达,与哮喘患者气道炎症指标密切相关,miR-30高表达会加重哮喘病情程度。

Objective To investigate the relationship between the expression of miR-30 in peripheral blood and airway inflammation indexes and the severity of asthma.Methods 80 patients with acute exacerbation of asthma admitted to our hospital between January 2021 and June 2023 were selected as the observation group as well as 80 patients from the population with health check-ups during the same period as the control group.The observation group was divided into mild group(n=28),moderate group(n=34),and severe group(n=18)based on the severity of the condition.The levels of miR-30 in peripheral blood and airway inflammation indexes[Transforming growth factorβ1(TGF-β1),Interleukin 6(IL-6),interleukin-33(IL-33),tumor necrosis factor-α(TNF-α)]were measured in the observation group and the control group.The expression of miR-30 in peripheral blood of patients with asthma and its correlation with airway inflammation were analyzed.Results Comparison of miR-30,TGF-β1,IL-6,IL-33,and TNF-αbetween the observation group and the control group,the difference was statistically significant(P<0.05).Comparison of miR-30,TGF-β1,IL-6,IL-33,TNF-α,eosinophilic granulocyte(EOS),and exhaled nitric oxide(FeNO)levels in the three groups of the mild,moderate,and severe groups,the difference was statistically significant(P<0.05).The correlation analysis showed a positive correlation between peripheral blood miR-30 and the levels of TGF-β1,IL-6,IL-33,and TNF-α(r>0,P<0.05),and Logistic regression analysis showed that peripheral blood miR-30,TGF-β1,TNF-α,FeNO,and EOS were the influencing factors for the degree of asthma disease condition(P<0.05).Conclusion Peripheral blood miR-30 exhibits abnormally high expression in asthma patients and is closely related to airway inflammation indicators.The high expression of miR-30 and the increase of airway inflammation index may aggravate the severity of asthma.High expression of miR-30 may exacerbate the severity of asthma.