宏基因二代测序对不明原因肺部感染的诊断价值

目的探讨宏基因二代测序在不明原因肺部感染患者上的诊断性能,以阐明宏基因二代测序的诊断价值和临床意义。方法选取2022年7月至2023年7月桐庐第一人民医院不明原因肺部感染患者34例,收集患者临床信息,比较传统病原体检测方法和宏基因检测的阳性率、感染类型检出和病原体分布。结果宏基因检测和传统病原体检测阳性率比较,差异有统计学意义(P<0.05)。宏基因检测出的细菌、病毒、真菌、支原体和衣原体种类和数量更多,同时混合感染或难培养病原体的检出率更高。胸痛患者宏基因阳性结果显著高于无胸痛患者(P<0.05)。结论相较于传统病原体检测,宏基因检测在不明原因肺部感染的患者上具有更好的诊断性能,尤其在混合感染和罕见或难培养病原体上。

Objective To explore the diagnostic performance of macro gene second generation sequencing on patients with unexplained lung infection,and to clarify the diagnostic value and clinical significance of macro gene second generation sequencing.Methods Thirty-four patients with unexplained lung infections in Tonglu First People's Hospital were selected from July 2022 to July 2023,and the clinical information of the patients was collected to compare the positivity rate,the detection of infection type and the distribution of pathogens between the traditional pathogen detection method and macro gene detection.Results The difference between the positive rate of macro gene and traditional pathogen detection was statistically significant(P<0.05).Macrogenes detected a greater number of bacterial,viral,fungal,mycoplasma,and chlamydial species and numbers,as well as a higher rate of detection of mixed infections and rare or difficult-to-cultivate pathogens.Macrogenic positive results were significantly higher in patients with chest pain than in those without chest pain(P<0.05).Conclusion Compared with traditional pathogen testing,macro genes have better diagnostic performance in patients with unexplained lung infections,especially in mixed infections and rare or difficult-to-cultivate pathogens.