不同遗传背景对水稻sdr4突变体表型的影响

Effects of Different Genetic Backgrounds on Phenotype of Rice sdr4 Mutants

SDR4基因是借助QTL(quantitative trait loci)方法克隆的调控水稻种子休眠的关键基因。种子休眠性状是由多基因调控的。利用CRISPR/Cas9技术敲除日本晴品种和宜香1B品种的SDR4基因,发现前者突变体完全无休眠,种子活力差;而后者突变体的种子呈弱休眠,有一定比例的穗发芽,但未穗发芽的种子可耐受脱水干燥。此外,可以明显观察到宜香1B背景下sdr4突变体的胚胎体积增加、胚胎蛋白含量增多,为研究SDR4基因功能及其下游基因,甚至克隆SDR4互作基因提供了重要基础。

The SDR4 gene is a key gene cloned by QTL(quantitative trait loci)method to regulate rice seed dormancy.The seed dormancy trait is regulated by multiple genes.This paper used CRISPR/Cas9 technology to knock out the SDR4 gene of Nipponbare and Yixiang 1B varieties,and found that the mutant of Nipponbare had no dormancy and poor seed vigor.The seeds of the mutant of Yixiang 1B were weakly dormant and had a certain percentage of pre harvest sprouting,but the seeds without pre-harvest sprouting could tolerate dehydration and drying.In addition,it could be clearly observed that the embryo volume and embryo protein content of sdr4 mutant increased under the background of Yixiang 1B,which provided an important foundation for studying the function of SDR4 gene and its downstream genes,and even cloning SDR4 interaction genes.