电解银离子杀灭艾滋病病毒的实验研究

Experimental Study on Inactivation of HIV by Electrolytic Silver Ion

目的探讨电解银离子体外灭活艾滋病病毒(HIV)的效果。方法用HIV-1病毒感染C8166细胞,并在C8166细胞中快速复制,然后释放到细胞培养上清中,使C8166细胞产生合胞体,当细胞密度达到1×10^(7)个·mL^(-1)时,用电解银离子与C8166细胞混合,室温孵育1 h后作为待测样品,在倒置显微镜下观察每孔中HIV-1ⅢB诱导的细胞病变效应,对细胞孔中的合胞体进行检测,使用Spearman-Karber法计算对照组和银离子灭火组的病毒TCID 50值。结果对照组的残余病毒滴度为106.67 TCID 50·mL^(-1),银离子灭活组残余病毒滴度为102.5 TCID 50·mL^(-1)。相对于对照组,银离子灭活组在室温1 h条件下对HIV-1病毒滴度的降低≥4.17 log,灭活率为99.993%。结论体外实验表明,电解银离子可有效杀灭HIV病毒,具备用于研制HIV病毒体外消杀用品的潜力。

Objective To investigate the in vitro efficacy of electrolyzed silver ions in inactivating human immunodeficiency virus(HIV).Methods HIV-1 was used to infect C8166 cells and rapidly replicate within them,with subsequent release into the cell culture supernatant,leading to the formation of syncytia in C8166 cells.When the cell density reached 1×10^(7) cells·mL^(-1),electrolyzed silver ions were mixed with the C8166 cells and incubated at room temperature for 1 hour.The resulting mixture was then used as the test sample.Under an inverted microscope,the cytopathic effect induced by HIV-1ⅢB in each well was observed,and syncytia within the wells were detected.The Spearman-Karber method was used to calculate the TCID 50 values of the virus in the samples.Results The residual virus titer in the control group was 106.67 TCID 50·mL^(-1),while that in the sample group was 102.5 TCID 50·mL^(-1).Compared with the virus control group,the sample group showed a reduction in HIV-1 virus titer of≥4.17 log units under room temperature conditions for 1 hour,corresponding to an inactivation rate of 99.993%.Conclusion In vitro experiments indicate that electrolyzed silver ions can effectively inactivate HIV,suggesting potential for use in the prevention and treatment of AIDS.