川芎-赤芍药对及其有效成分抑制ox-LDL诱导的巨噬细胞泡沫化及铁调素的作用机制

Mechanism of Chuanxiong-Chishao and its Effective Ingredients on ox-LDL-induced Macrophage Foaming and Hepcidin

目的:观察川芎-赤芍药对及其有效成分能否减缓细胞泡沫化,并探讨其作用机制。方法:采用氧化型低密度脂蛋白(ox-LDL)诱导人单核细胞白血病(THP-1)细胞建立泡沫化模型,将川芎-赤芍药对及其有效成分分别作用于ox-LDL诱导的THP-1细胞。采用油红O染色分析细胞泡沫化程度,通过逆转录定量聚合酶链反应(RT-qPCR)检测铁代谢相关铁调素(Hep)、膜铁转运蛋白(FPN)、铁调素调节蛋白(HJV)、血色素沉着症基因(HFE)、转铁蛋白受体2(TfR2)mRNA表达情况。结果:油红O细胞染色后镜下可见,染色后的红色脂滴因川芎-赤芍药对及其有效成分干预而减少。与对照组比较,模型组Hep、FPN、HFE、TfR2 mRNA表达上升,HJV mRNA表达减少;与模型组比较,川芎-赤芍药对及其有效成分干预组抑制作用明显,差异均有统计学意义(P<0.05或P<0.01)。结论:川芎-赤芍药对及其有效成分可抑制ox-LDL诱导的THP-1源巨噬细胞泡沫化,作用机制与川芎-赤芍药对及其有效成分调节铁代谢相关基因表达有关。

Objective:To observe the effect of Chuanxiong-Chishao and its effective ingredients on cell foaming,and to explore its mechanism of action.Methods:The foaming model of THP-1 cells was established by oxidized low density lipoprotein(ox-LDL).The tohoku hospital pediatrics-1(THP-1)cells induced by ox-LDL were treated with Chuanxiong-Chishao and its effective ingredients respectively.The cell foam was analyzed by oil red O staining,and the mRNA expressions of hepcidin(Hep),ferroportin(FPN),ferrimodulin regulatory protein(HJV),hemochromatosis gene(HFE)and transferrin receptor 2(TfR2)were detected by reverse transcription quantitative polymerase chain reaction(RT-qPCR).Results:Oil red O staining showed that the number of red fat drops decreased with the intervention of Chuanxiong-Chishao and its effective ingredients.Compared with the control group,the mRNA expressions of Hep,FPN,HFE and TfR2 increased in the model group,while mRNA expression of HJV decreased.Compared with the model group,Chuanxiong-Chishao and its effective ingredients of the intervention group showed obvious inhibitory effect,and the differences were statistically significant(P<0.05 or P<0.01).Conclusion:Chuanxiong-Chishao and its effective ingredients could inhibit the ox-LDL-induced THP-1-derived macrophage foaming,and its mechanism of action was related to the regulation of iron metabolism related gene expression.