低温胁迫下厌氧氨氧化系统微生物群落相互作用关系研究

Study of microbial community interactions in anaerobic ammonia oxidation system under low temperature stress

本文探究了梯度降温(30~5℃)过程中Anammox系统的脱氮性能、群落结构、功能基因组成及微生物间的相互作用关系.结果表明,25~15℃的低温促进了EPS的分泌,提升了群落的丰富度和多样性,总体上看群落结构相对稳定,在降温至5℃时,系统脱氮性能良好.降温过程对Anammox代谢相关基因丰度的抑制作用不明显,hao可以替代AMX1缺失的nir提供NO进行Anammox代谢.此外,基于宏基因组测序数据进行Binning分析获得了23个宏基因组组装基因组(MAGs),其中存在基因缺陷的AMX1通过与伴生菌进行氮代谢互养、碳代谢互养、EPS代谢互养及二次代谢产物代谢互养来完成各种代谢活动.

In this study,nitrogen removal performance,community structure,functional gene composition,and microbial interactions were investigated in Anammox system during gradient cooling process(30~5℃).25~15℃promoted the secretion of EPS and stimulated the abundance and diversity of the microbial community,while the community structure is relatively stable.And the system performance remained well when the temperature dropped to 5℃.The inhibitory effect of the cooling process on the abundance of genes related to Anammox metabolism was not obvious,and hao could replace AMX1-deficient nir to provide NO for Anammox metabolism.In addition,binning analysis based on metagenomics data obtained 23 metagenomic assembly genomes(MAGs),and it was found that genetically defective AMX1 could perform various metabolic activities through metabolic interaction with other bacteria in Anammox consortia about nitrogen metabolism,carbon metabolism,EPS metabolism,as well as secondary metabolite metabolism.