振荡-静置循环培养新策略提升灵芝三萜合成与菌丝体活性

Oscillation-static cycle cultivation enhances the synthesis of triterpenes and mycelium activity in Ganoderma lucidum

灵芝作为历史悠久的食药两用型真菌,其药用价值主要源自灵芝三萜这一主要活性物质,灵芝三萜具有抗肿瘤、抗氧化等药理活性。本研究旨在建立高效液态发酵生产灵芝三萜的技术体系。针对传统深层发酵和振荡-静置两阶段培养的局限性,利用振荡-静置循环培养工艺,结合遗传算法构建人工神经网络模型进行优化。在优化条件下,液态发酵高产灵芝三萜的最佳培养方式为振荡2.8 d-静置7.3 d-振荡0.2 d-静置0.3 d。此条件下,灵芝三萜含量达到20.82 mg/g,灵芝酸得率为129.09 mg/L,与Z10J0相比提高了324.78%,且培养周期缩短至10.6 d。同时,该培养方式下菌丝体具有较好的抗肿瘤活性和抗氧化活性。本研究开发了一种经济有效的液态发酵培养方式,可简化工艺流程、缩短发酵周期并有效改善传统培养方式的弊端;同时为液态发酵高产灵芝三萜的规模化应用提供了参考,具有广阔的应用前景。

Ganoderma lucidum is a precious fungus with both edible and medicinal values and has a long history of medical use.Triterpenes as the main active components endow G.lucidum with anti-tumor,antioxidant,and other pharmacological activities.The present study endeavors to establish a proficient liquid-state fermentation technology for the enhanced production of triterpenes.In view of the limitations inherent in conventional submerged fermentation and oscillation-static two-stage cultivation,this study established an oscillation-static cycle cultivation process and optimized the cultivation conditions by building an artificial neural network model based on genetic algorithms.The cultivation conditions for the high-yield production of triterpenes were optimized as follows:2.8 days of oscillation,7.3 days of static cultivation,0.2 day of oscillation,and 0.3 day of static cultivation.Under these conditions,the content of triterpenes reached 20.82 mg/g.The yield of triterpenes reached 129.09 mg/L,showing a remarkable increase of 324.78%compared with that of the Z10J0 method.Moreover,the established method shortened the cultivation cycle by 10.6 days.The mycelia cultivated under this regimen exhibited commendable anti-tumor and antioxidant activities.This study not only presents an economical liquid-state fermentation approach but also streamlines the fermentation flow,reduces fermentation duration,and effectively ameliorates drawbacks associated with conventional cultivation methods.In addition,this study gives valuable insights into the scaled application of liquid-state fermentation in the high-yield production of triterpenes,which showcases broad prospects.