miR-126及Th1相关细胞因子在非小细胞肺癌患者血清中的表达水平及临床意义

The expression and clinical significance of miR⁃126 and Th1 related cytokines in the serum of non⁃small cell lung cancer patients

目的探讨miR-126及Th1相关细胞因子在非小细胞肺癌(NSCLC)患者血清中的表达水平,及其与肿瘤标志物水平和临床病理特征的关系。方法选取自贡市第一人民医院2020年4月-2023年3月收治的NSCLC患者102例为研究组,并以同期健康体检人员102名为对照组。检测比较两组miR-126、Th1相关细胞因子[白细胞介素(IL)-2、干扰素(IFN)-γ、肿瘤坏死因子(TNF)-α]、肿瘤标志物[癌胚抗原(CEA)、糖类抗原(CA)199、CA125、细胞角蛋白19可溶性片段(CYFRA)]水平。统计研究组患者的TNM分期、淋巴结转移等临床病理特征,并比较miR-126、Th1相关细胞因子、肿瘤标志物在不同临床病理特征NSCLC患者中的水平差异。采用Pearson相关法分析NSCLC患者miR-126、Th1相关细胞因子与肿瘤标志物水平的关系。结果研究组血清miR-126和IL-2水平低于对照组(t=15.816、81.256),血清IFN-γ、TNF-α、CEA、CA199、CA125、CYFRA水平均高于对照组(t=39.166、30.190、62.488、24.722、58.627、27.539),差异均有统计学意义(P均<0.05)。TNMⅢ、Ⅳ期患者的血清miR-126和IL-2水平低于Ⅰ、Ⅱ期患者(t=6.060、5.509),血清IFN-γ、TNF-α、CEA、CA199、CA125、CYFRA水平均高于Ⅰ、Ⅱ期患者(t=10.944、8.196、11.027、11.603、10.384、7.566),差异均有统计学意义(P均<0.05)。发生淋巴结转移患者的血清IFN-γ、TNF-α、CEA、CA125、CYFRA水平均高于无淋巴结转移患者,差异均有统计学意义(t=8.951、6.414、8.852、8.690、7.224,P均<0.05)。Pearson相关分析结果显示,NSCLC患者血清miR-126、IL-2、IFN-γ、TNF-α水平与其血清CEA、CA199、CA125、CYFRA水平均密切相关(P<0.05)。结论NSCLC患者miR-126及Th1相关细胞因子水平与肿瘤标志物水平和临床病理特征相关,可能可以作为NSCLC病情判断参考指标。

Objective To investigate the expression levels of miR⁃126 and Th1 related cytokines in the serum of non⁃small cell lung cancer(NSCLC)patients and their relationship with tumor marker levels and clinical pathological characteristics.Methods A total of 102 NSCLC patients admitted to the Zigong First People's Hospital from April 2020 to March 2023 were selected as the study group,and 102 health examination people during the same period were selected as the control group.The levels of miR⁃126,Th1 related cytokines[interleukin(IL)⁃2,interferon(IFN)⁃γ,tumor necrosis factor(TNF)⁃α],tumor markers[carcinoembryonic antigen(CEA),carbohydrate antigen(CA)199,CA125,cytokeratin 19 soluble fragment(CYFRA)]of the two groups were detected and compared.Study group patients'TNM staging,lymph node metastasis,and other clinical and pathological characteristics were statistically analyzed.The levels of miR⁃126,Th1 related cytokines,and tumor markers in NSCLC patients with different clinical and pathological characteristics were compared.Pearson correlation method was used to analyze the relationship between miR⁃126,Th1 related cytokines with tumor marker levels in NSCLC patients.Results The levels of serum miR⁃126 and IL⁃2 in the study group were significantly lower than those in the control group(t=15.816,81.256),while the levels of serum IFN⁃γ,TNF⁃α,CEA,CA199,CA125 and CYFRA were significantly higher than those in the control group(t=39.166,30.190,62.488,24.722,58.627,27.539),the differences were statistically significant(all P<0.05).The levels of serum miR⁃126 and IL⁃2 in patients with TNM stageⅢandⅣwere significantly lower than those in patients with TNM stageⅠandⅡ(t=6.060,5.509),while the levels of serum IFN⁃γ,TNF⁃α,CEA,CA199,CA125 and CYFRA were significantly higher than those in patients with TNM stageⅠandⅡ(t=10.944,8.196,11.027,11.603,10.384,7.566),the differences were statistically significant(all P<0.05).The levels of serum IFN⁃γ,TNF⁃α,CEA,CA125 and CYFRA in patients with lymph node metastasis were significantly higher than those in patients without lymph node metastasis,the differences were statistically significant(t=8.951,6.414,8.852,8.690,7.224;all P<0.05).Pearson correlation analysis showed that the levels of serum miR⁃126,IL⁃2,IFN⁃γand TNF⁃αin NSCLC patients were closely related to the levels of serum CEA,CA199,CA125 and CYFRA(P<0.05).Conclusion The levels of miR⁃126 and Th1 related cytokines in NSCLC patients were correlated with tumor marker levels and clinical pathological characteristics,which might serve as reference indicators for the disease severity diagnosis of NSCLC.