麻花艽内生真菌群落多样性及土壤理化因子相关性研究

Correlation between the Diversity of Endophytic Fungal Communities in Gentiana Straminea Maxim.and Soil Physicochemical Factors

本文以高寒草甸的麻花艽(Gentiana straminea Maxim.)为研究对象,采用组织块培养法对麻花艽根、花、茎和叶四个组织部位的内生真菌进行分离,经形态学及ITS测序鉴定后分析麻花艽内生真菌的多样性。结果显示,麻花艽不同组织部位中共获得268株内生真菌,共聚类为40个OTUs。根、花、茎和叶部位的较优势菌属依次为刚毛藻属(Cladophora),灰葡萄孢属(Botrytis),枝孢菌属(Cladosporium)和壳针孢菌属(Septoria)。内生真菌群落多样性指数(Shannon指数、Simpson指数)均为根部较高。根和茎部位内生真菌群落结构相近,差异较小。土壤pH、全氮、全钾、速效磷和容重是麻花艽内生真菌群落多样性的主要影响因子。

The endophytic fungi of Gentiana straminea Maxim.from alpine meadows were isolated from four tissue parts:roots,flowers,stems and leaves by using tissue block culture method.The diversity of endophytic fungi of G.straminea was analysed after morphological and ITS sequencing identification.As the results showed,a total of 268 endophytic fungal strains were obtained from different tissue parts of G.straminea,which were classified into 40 OTUs.In the order of more dominant genera in the root,flower,stem and leaf parts were Cladophora,Botrytis,Cladosporium and Septoria.The endophytic fungal community diversity indices(Shannon's index,Simpson's index)were all higher in the roots.The endophytic fungi community structure in the root and stem parts was similar and less different.Soil pH,total nitrogen,total potassium,quick-acting phosphorus and bulk weight were the main factors influencing the diversity of endophytic fungal communities of G.straminea.