融化后的FFP、新鲜液体血浆在2~6℃环境中储存凝血功能等指标的变化

Changes of coagulation function and other indicators of the thawed FFP and FLP at 2-6℃

目的观察融化后新鲜冰冻血浆、新鲜液体血浆在2~6℃储存过程中部分凝血因子活性和蛋白含量的变化,为探索FFP解冻后2~6℃储存的合适时间提供参考。方法小包装/大包装冻融组新鲜冰冻血浆分别于融化后d1、2、3、4、5、6、7检测凝血因子FⅤ活性(FⅤ∶C)、凝血因子FⅧ活性(FⅧ∶C)、纤维蛋白原(Fib)、总蛋白(TP)、白蛋白(Alb)的含量;新鲜液体血浆分别于制备后d1、2、3、4、5、6、7及制备后d1、6、11、16、21、26、31检测FⅤ∶C、FⅧ∶C、Fib、TP、Alb的含量。结果FFP组FⅧ∶C随着融化后储存时间的延长逐渐下降(P<0.05),两组在d7分别下降了37.4%和47.6%;FⅤ∶C、Fib、TP、Alb差异无统计学意义(P>0.05)。新鲜液体血浆组FⅧ∶C随着融化后储存时间的延长逐渐下降(P<0.05);FⅤ∶C储存7 d组差异无统计学意义(P>0.05),储存31d组逐渐下降(P<0.05);Fib、TP、Alb差异无统计学意义(P>0.05)。结论2~6℃储存7 d的融化FFP虽然FⅧ∶C有所下降,但稳定在52.4%,符合临床需求。

Objective To observe the changes of coagulation factor activity and protein content of the thawed fresh frozen plasma(FFP)and fresh liquid plasma(FLP)during storage at 2-6℃,and to provide reference for exploring the appropriate storage time of FFP at 2-6℃after thawing.Methods The small-thaw group and the large-thaw group were respectively detected for the activity of coagulation factor FⅤ(FⅤ∶C)and FⅧ(FⅧ∶C),and the levels of fibrinogen(Fib),total protein(TP)and albumin(Alb)in TTP at 1,2,3,4,5,6 and 7 days after thawing.And the FLP was detected for FⅤ∶C,FⅧ∶C,Fib,TP and Alb at 1,2,3,4,5,6,7 days and 1,6,11,16,21,26 and 31 days after preparation,respectively.Results In FFP group,FⅧ∶C decreased gradually with the prolongation of storage time after melting(P<0.05),and decreased by 37.4%and 47.6%respectively in the two groups on the 7th day.There was no statistical difference in FⅤ∶C,Fib,TP and Alb(P>0.05).In FLP group,FⅧ∶C decreased gradually with the prolongation of storage time after melting(P<0.05).There was no statistical difference in FⅤ∶C in 7-day storage group(P>0.05),but it decreased gradually in 31-day storage group(P<0.05).There was no statistical difference in Fib,TP and Alb(P>0.05).Conclusion Although FⅧ∶C is decreased in thawed FFP stored at 2-6℃for 7 days,it is still about 52.4%,which should be able to play a normal role in clinical practice.