固定化脱氮菌及COD降解菌协同作用对养殖污水处理效果的分析

Analysis of the Synergistic Effect of Immobilized Denitrification Bacteria and COD Degrading Bacteria on the Treatment of Aquaculture Wastewater

本研究利用从猪场污水中分离、筛选和鉴定的异养硝化细菌(Acinetobacter bereziniae Zhi77),通过氯化钙(CaCl_(2))和海藻酸钠(C6H7NaO6)固定后,获得固定化硝化细菌,分析培养温度、菌株添加量和菌株反应时间等因素对固定化菌株脱氮效果的影响。结果表明,固定化硝化细菌在30℃,菌株添加量2 mL,反应培养时间12 d条件下,氨氮去除率达到95.73%,总氮去除率达到80.43%。在COD降解菌和固定化异养硝化细菌的协同作用下,投加微生物且曝气的处理组,污水中化学需氧量(COD)、氨氮(NH4-N)、总氮(TN)、悬浮物(SS)和总磷(TP)去除率明显升高,分别达到87.90%、96.08%、94.50%、82.70%和23.64%。猪场污水中各项污染物浓度明显下降,该研究结果在畜禽养殖场污水处理领域具有良好的应用前景。

In this paper,heterotrophic nitrifying bacteria(Acinetobacter bereziniae Zhi77)was isolated,screened and identified from pig farm sewage.Immobilized nitrifying bacteria were obtained by calcium chloride(CaCl_(2))and sodium alginate(C_(6)H_(7)NaO_(6))reagents.The effects of culture temperature,strain addition and strain reaction time on the nitrogen removal efficiency of immobilized strains were analyzed.The results showed that the removal rates of ammonia nitrogen and TN reached 95.73%and 80.43%,respectively,under the condition of 30℃,2 mL strain addition and 12 days of culture.Under the synergistic effect of COD degrading bacteria and immobilized heterotrophic nitrifying bacteria,the removal rates of COD,NH_(4)-N,TN,SS and TP in the treatment group with microbial addition and aeration were significantly increased,reaching 87.90%,96.08%,94.50%,82.70%and 23.64%,respectively.The concentrations of various pollutants in the pig farm sewage were significantly decreased,which has good application prospects in the field of livestock farm sewage treatment.