巴戟天多糖对文昌鸡生长性能、血清免疫和抗氧化指标及免疫器官发育的影响

Effects of Morinda officinalis Polysaccharide on Growth Performance,Serum Immune and Antioxidant Indexes and Immune Organs Development of Wenchang Chickens

【目的】本试验通过研究饲粮中添加巴戟天多糖(Morinda officinalis polysaccharide,MOP)对文昌鸡生长性能、血清免疫和抗氧化指标及免疫器官发育的影响,旨在为MOP在肉鸡生产中得到深入应用提供参考依据。【方法】选取6日龄体重相近、健康的雌性文昌鸡600只,随机分为5组,每组8个重复,每个重复15只鸡,对照组鸡饲喂基础饲粮,试验组鸡分别在基础饲粮中添加250、500、1000和2000 mg/kg的MOP,试验期40 d。在试验第1、40天分别称重,试验期间以重复为单位记录采食量和剩料量,计算平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G);在试验第40天每重复选取1只试验鸡采血,屠宰后采集胸腺、脾脏、法氏囊并称重,测定血清生化指标、抗氧化指标和免疫指标,并测定免疫器官指数。【结果】与对照组相比,①饲粮中添加不同水平的MOP对文昌鸡的终末体重、ADG和ADFI无显著影响(P>0.05),但250 mg/kg MOP组的F/G显著提高(P<0.05),其余试验组F/G无显著变化(P>0.05)。②250 mg/kg MOP组文昌鸡血清免疫球蛋白G(IgG)含量、500 mg/kg MOP组血清IgM含量均显著提高(P<0.05);500和1000 mg/kg MOP组血清丙二醛(MDA)含量显著降低(P<0.05);2000 mg/kg MOP组血清谷胱甘肽过氧化物酶(GSH-Px)活性显著提高(P<0.05)。③500、1000和2000 mg/kg MOP组文昌鸡法氏囊指数均显著提高(P<0.05)。【结论】饲粮中添加250及500 mg/kg MOP可提高文昌鸡血清免疫球蛋白含量,添加500、1000和2000 mg/kg MOP可改善文昌鸡血清抗氧化指标,促进免疫器官法氏囊的生长发育。本试验中,MOP添加量以500 mg/kg为宜。

【Objective】This experiment was conducted to investigate the effects of Morinda officinalis polysaccharides(MOP)on growth performance,serum immune and antioxidant function and immune organs development of Wenchang chickens,in order to provide reference for the application of MOP in healthy breeding of broilers.【Method】Six hundred 6-day-old healthy female Wenchang chickens were selected and randomly divided into 5 groups,with 8 replicates in each group and 15 chickens in each replicate.The chickens in control group was fed with basal diet,and the chickens in the experiment groups were fed with basal diet supplemented with 250,500,1000 and 2000 mg/kg MOP,respectively.The experiment lasted for 40 days.Body weights were taken on the 1st and 40th day of the trial to calculate average daily gain(ADG).During the trial period,the leftover feed was recorded in repetition,and average daily feed intake(ADFI)and feed-to-gain ratio(F/G)were calculated.On the 40th day of the trial,one chicken from each replicate was selected for blood sampling and tissue collection.Serum biochemical indicator,antioxidant indicator,immune indicator and immune organ index of Wenchang chickens were determined.【Result】Compared with control group,①Adding different levels of MOP had no significant effects on the final body weight,ADG and ADFI of Wenchang chickens(P>0.05),but F/G of 250 mg/kg MOP group was significantly increased(P<0.05),and F/G of other experiment groups did not have significant changes(P>0.05).②The serum IgG content in 250 mg/kg MOP group and the serum IgM content in 500 mg/kg MOP group were significantly increased(P<0.05),serum MDA contents in 500 and 1000 mg/kg MOP groups were significantly reduced(P<0.05),and serum GSH-Px activity in 2000 mg/kg MOP group was significantly increased(P<0.05).③Dietary addition of 500,1000 and 2000 mg/kg MOP could significantly increase the bursa of Fabricius index of Wenchang chickens(P<0.05).【Conclusion】The addition of 250 and 500 mg/kg MOP in this experiment could increase the serum immunoglobulin content of Wenchang chickens,and the addition of 500,1000 and 2000 mg/kg MOP could improve the serum antioxidant indicator of Wenchang chickens and promote the growth and development of the bursa of Fabricius.In this experiment,the appropriate amount of MOP addition was 500 mg/kg.