大蒜素缓解伏马毒素B_(1)致鹌鹑肝损伤的研究

Study on Allicin Alleviating the Liver Injury of Quail Caused by Fumonisin B_(1)

【目的】探索大蒜素缓解伏马毒素B_(1)(FB_(1))造成产蛋期鹌鹑肝脏损伤的机制,为预防FB_(1)诱导鹌鹑肝损伤提供试验依据,减少FB_(1)中毒给养殖业造成的经济损失。【方法】以黄羽雌性鹌鹑为试验对象,将120只鹌鹑随机分为4组,分别为对照组、大蒜素组(500 mg/kg大蒜素)、伏马毒素组(30 mg/kg FB_(1))及缓解组(500 mg/kg大蒜素+30 mg/kg FB_(1)),每组30只。观察鹌鹑肝脏组织病理学及组织超微结构变化,并检测肝功能、抗氧化功能指标及相关炎症因子的表达。【结果】肝脏病理学与超微结构结果显示,大蒜素能够有效缓解FB_(1)造成的鹌鹑肝脏线粒体嵴消失、胶原纤维增加等病理损伤。与对照组相比,伏马毒素组鹌鹑血清中谷草转氨酶(AST)浓度显著升高(P<0.05);过氧化氢酶(CAT)活力、总抗氧化物能力(T-AOC)均显著降低(P<0.05),丙二醛(MDA)含量显著升高(P<0.05);白细胞介素8(IL-8)、IL-6、IL-18、IL-12β、肿瘤坏死因子α(TNF-α)、环氧化酶2(COX-2)、诱导型一氧化氮合酶(iNOS)、相关凋亡因子配体(FASL)及Toll样受体4(TLR4)基因表达量均显著升高(P<0.05)。与伏马毒素组相比,缓解组鹌鹑血清中AST含量显著降低(P<0.05);CAT活力、T-AOC显著升高(P<0.05),MDA含量显著降低(P<0.05);IL-8、IL-6、IL-18、IL-12β、TNF-α、COX-2、iNOS、FASL、TLR 4基因表达量均显著降低(P<0.05)。【结论】大蒜素可有效缓解FB_(1)导致的鹌鹑肝损伤,其机制与大蒜素的抗炎作用和抗氧化作用密切相关。

【Objective】The objective of this experiment was to explore the mechanism of allicin in alleviating liver injury caused by fumonisin B_(1)(FB_(1))in quail during egg-laying period,so as to provide experimental basis for the prevention of FB_(1)-induced liver injury in quail,and reduce the economic loss caused by FB_(1)poisoning to the breeding industry.【Method】The Yellow-feathered female quails were used as test subjects,and 120 quails were randomly divided into 4 groups:Control group,allicin group(500 mg/kg allicin),fumonisin group(30 mg/kg FB_(1)),and remission group(500 mg/kg allicin+30 mg/kg FB_(1)),with 30 quails in each group.The histopathological and ultrastructural changes of liver in quails were observed,the indexes of liver function,antioxidant function and the expression of related inflammatory factors in quail were detected.【Result】The results of liver pathology and ultrastructure showed that allicin could effectively alleviate the pathological damage caused by FB_(1),such as the disappearance of mitochondrial cristae and the increase of collagen fibers.Compared with control group,the concentration of aspartate aminotransferase(AST)in serum of quails in fumonisin group was significantly increased(P<0.05),the activitiy of catalase(CAT)and total antioxidant capacity(T-AOC)were significantly decreased(P<0.05),and the malondialdehyde(MDA)content was significantly increased(P<0.05).The expressions of interleukin-8(IL-8),IL-6,IL-18,IL-12β,tumor necrosis factor-α(TNF-α),cyclooxygenase-2(COX-2),inducible nitric oxide synthase(iNOS),related apoptosis factor ligand(FASL)and Toll-like receptor 4(TLR4)genes were also significantly increased(P<0.05).Compared with fumonisin group,the AST content in serum of quails in remission group was significantly decreased(P<0.05),the activitiy of CAT and T-AOC were significantly increased(P<0.05),and MDA content was decreased significantly(P<0.05).The expressions of IL-8,IL-6,IL-18,IL-12β,TNF-α,COX-2,iNOS,FASL and TLR 4 genes were also significantly decreased(P<0.05).【Conclusion】Allicin could effectively alleviate FB_(1)-induced liver injury in quails,and its mechanism was closely related to the anti-inflammatory and antioxidant effects of allicin.