不同产地三叶木通籽油脂肪酸指纹图谱的构建

Construction of Fatty Acid Fingerprint of Akebia trifoliata Seed Oil from Different Origins

为构建三叶木通籽油脂肪酸指纹图谱,探索不同地区三叶木通籽油的脂肪酸差异特征指标,采用索氏抽提法提取来自不同产地共计18批次样品,分析其脂肪酸组成和含量,通过“中药色谱指纹图谱相似度评价系统”建立三叶木通籽油脂肪酸指纹图谱,结合主成分和PLS-DA初步分析具差异特征指标。结果表明,三叶木通籽油的主要脂肪酸是油酸、亚油酸和棕榈酸,其相对含量均超过19%,建立了三叶木通籽油气相色谱标准指纹图谱,样品与标准指纹图谱的相似度在0.994以上,准确性和可靠度较高。以油酸、亚油酸和棕榈酸作为差异标志物可对产地归属初步判别,但仍需要结合更加多元的特征结果进行分析和判定。

In order to establish a fatty acid fingerprint of the seed oil from Akebia trifoliata and explore the differential characteristic indicators of fatty acid composition in the seed oil from different regions,a total of 18 batches of Akebia trifoliata seed oil from different regions were extracted using Soxhlet extraction method.The fatty acid composition and content were analyzed,and the Akebia trifoliata seed oil fatty acid fingerprint was established using the "Chinese medicine chromatographic fingerprint similarity evaluation system".Principal component analysis(PCA) and partial least squares discriminant analysis(PLS-DA) were performed to analyze the differential characteristic indicators of Akebia trifoliata seed oil.The results indicated that,the main fatty acids in Akebia trifoliata seed oil were oleic acid,linoleic acid,and palmitic acid,with their relative contents exceeding 19%.The fatty acid fingerprint of Akebia trifoliata seed oil was established,the similarity between the samples and the standard fingerprint was above 0.994,indicating the high accuracy and reliability.Oleic acid,linoleic acid,and palmitic acid can be used as differential markers for preliminary discrimination of geographical origin.However,further analysis and determination should be done by considering more diverse characteristic results.