室温挤出3D打印HA/β-TCP/DCPA骨支架的复合浆料研究

Composite Slurry for Room-Temperature Extrusion 3D Printing HA/β-TCP/DCPA Bone Scaffold

基于3D打印骨支架的骨组织工程是一种具有应用潜力的骨修复策略。本文将植酸螯合改性的羟基磷灰石(IP6-HA)、β-磷酸三钙(β-TCP)与无水磷酸二氢钙(MCPA)按照不同摩尔比混合的复合粉体作为固相,以不同丙三醇(甘油)含量的水溶液作为液相,通过调整复合粉体中IP6-HA粉末的含量与固化液中甘油的含量,制备了能够应用于室温挤出3D打印骨支架的透钙磷石体系磷酸钙复合浆料。结果表明:复合浆料的水化产物主要为HA、β-TCP和无水磷酸氢钙(DCPA);当固化液中甘油含量为50%(质量分数)时,复合浆料均表现出优异的可打印性能与力学性能,抗压强度最高可达19.8 MPa;残余IP6-HA的存在改变了固化样品表面磷灰石的矿化结构并提升了矿化能力;制备的支架结构完整,孔洞均匀,抗压强度可达5.8 MPa。本研究制备的磷酸钙复合浆料能够通过室温挤出式3D打印技术制备骨支架,在3D打印领域具有良好的应用前景。

Bone tissue engineering based on 3D printing bone scaffolds is a potential bone repair strategy.The composite powders of phytic acid chelating modified hydroxyapatite(IP6-HA),β-tricalcium phosphate(β-TCP) and anhydrous calcium dihydrogen phosphate(MCPA) with different molar ratios were used as solid phase,and the aqueous solution with different propylene glycol(glycerine) content was used as liquid phase.By adjusting IP6-HA powder content in composite powders and glycerine content in the curing solution,the calcium phosphate composite slurries of brushite system that can be applied to the room-temperature extrusion 3D printing bone scaffolds were prepared.The results show that hydration products of the composite slurries are mainly hydroxyapatite(HA),β-TCP and anhydrous calcium hydrogen phosphate(DCPA).When the glycerine content in the curing solution is 50%(mass fraction),the composite slurries exhibit excellent printability and mechanical properties,and the compressive strength can reach up to 19.8 MPa.The presence of residual IP6-HA changes the mineralization structure of apatite on surface of the solidified samples and improves the mineralization ability.The prepared scaffolds have a complete structure and uniform pores,and the compressive strength can reach 5.8 MPa.The composite slurries prepared in this study can be used to prepare bone scaffolds by room-temperature extrusion 3D printing technology,and has a good application prospect in the field of 3D printing bone scaffolds.