非血缘供者外周血造血干细胞采集效果及其影响因素分析

Effect and Influencing Factors of Peripheral Blood Hematopoietic Stem Cells Collection from Unre lated Donors

目的:分析非血缘供者外周血造血干细胞采集效果并探讨其影响因素。方法:对2021年1月至2023年12月岳阳市中心医院113例非血缘供者的外周血造血干细胞动员及采集相关结果进行回顾性分析。结果:113例供者均成功动员采集,干细胞采集物中MNC数为(12.40±7.41)×10^(8)/kg、CD34^(+)细胞数为(10.64±8.07)×10^(6)/kg。单因素分析结果显示,男性供者的干细胞采集物CD34^(+)细胞比例明显高于女性供者(P=0.015),采集前白细胞计数与干细胞采集物有核细胞计数呈正相关(r=0.388),供者体重、采集前外周血CD34^(+)细胞比例与干细胞采集物CD34^(+)细胞比例呈正相关(r=0.259,r=0.780),供者rhG-CSF日剂量与干细胞采集物CD34^(+)细胞比例呈负相关(r=-0.285)两种动员剂(惠尔血和格拉诺赛特)均取得良好动员采集效果。多因素分析显示,采集前外周血白细胞计数是干细胞采集物有核细胞计数的影响因素(P <0.001),采集前外周血CD34^(+)细胞比例是干细胞采集物CD34^(+)细胞比例的影响因素(P<0.001)。结论:非血缘供者外周血造血干细胞动员、采集效果良好,采集前外周血白细胞计数和CD34^(+)细胞比例是预测采集效果的较可靠指标。

Objective:To analyze the effect of peripheral blood hematopoietic stem cells(PBSC)collection from unrelated donors and its influencing factors.Methods:A retrospective analysis was conducted on the mobilization and collection of PBSC from 113 unrelated donors at Yueyang Central Hospital from January 2021 to December 2023.Results:113 donors were successfully mobilized.The average count of PBSC mononuclear cells(MNC)and CD34^(+) cells were(12.40±7.41)×10^(8)/kg and(10.64±8.07)×10^(6)/kg,respectively.Univariate analysis showed that the PBSC CD34^(+)cells ratio of male donors was significantly higher than that in female donors(P=0.015).The peripheral blood(PB)white blood cell(WBC)count before collection was positively correlated with the PBSC nucleated cells count(r=0.388),and the donor's body weight,the PB CD34^(+)cell ratio before collection were positively correlated with the PBSC CD34^(+)cell ratio(r=0.259,r=0.780).The daily dose of thG-CSF was negatively correlated with the PBSC CD34^(+)cell ratio(r=-0.285).Both thG-CSF agents achieved successful mobilization.Multivariate analysis showed that PB WBC count before collection was a factor affecting the count of PBSC nucleated cells(P<0.001),while the PB CD34^(+)cell ratio before collection was a factor affecting the PBSC CD34^(+)cell ratio(P<0.001).Conclusion:The mobilization and collection of PBSC from unrelated donors are good,and the PB WBC count and CD34^(+)cell ratio before collection are reliable indicators for predicting the collection effect.