异荭草素-玉米醇溶蛋白/阿拉伯胶纳米纤维膜的制备表征及其愈伤作用

The effect of isoorientin-zein/Arabic gum composite nanofiber membrane on wound healing in mice

为探究异荭草素(isoorientin,ISO)与玉米醇溶蛋白(zein)/阿拉伯胶(Arabic gum,GA)制得的复合纳米纤维膜对小鼠皮肤损伤愈合的影响,以空白纺丝膜为对照,分别使用负载zein/GA的纳米纤维膜、负载ISO的纳米纤维膜以及负载ISO-zein/GA的复合纳米纤维膜对小鼠伤口进行敷料处理。结果表明,负载zein/GA的纳米纤维膜、负载ISO的纳米纤维膜以及负载ISO-zein/GA的复合纳米纤维膜对小鼠的伤口愈合具有促进作用,可以有效减少伤口氧化损伤及促炎因子TNF-α、IL-1β、iNOS的mRNA表达水平,并促进伤口血管重建,从而加速伤口愈合。研究结果验证了异荭草素-玉米醇溶蛋白/阿拉伯胶复合纳米纤维膜对伤口愈合的促进作用,拓宽了异荭草素的应用范围,为新型伤口敷料的研究提供了思路。

To investigate the effect of composite nanofiber membrane prepared from isoorientin(ISO)and zein/Arabic gum(GA)on skin injury healing in mice,this study used blank spinning membrane as control,and wound dressings were applied to mice using zein/GA loaded nanofiber membrane,ISO loaded nanofiber membrane,and ISO-zein/GA loaded composite nanofiber membrane,respectively.The results showed that the nanofiber membranes loaded with zein/GA,ISO and ISO-zein/GA had promoting effects on wound healing in mice.They effectively reduced wound oxidative damage and mRNA expression levels of pro-inflammatory factors(TNF-α、IL-1β、iNOS),and promoted wound vascular reconstruction,thereby accelerating wound healing.The research results verify the promoting effect of ISO-zein/GA composite nanofiber membrane on wound healing,expand the application range of isoorientin,and provide ideas for the research of new wound dressing.