摘要
利用麦类壳多胞斑点病菌β-tubulin一段保守基因序列,设计特异性引物进行麦类壳多胞斑点病菌的检测,并进行特异性与灵敏度验证。结果显示,该方法特异性强,灵敏度可达5 pg/μL,适合于麦类壳多胞斑点病菌的快速检测。
This study utilized partly conserved gene sequence ofβ-tubulin in Parastagonospora pseudonodorum,designed specific primers for the detection of Parastagonospora pseudonodorum,with specificity and sensitivity verified.The results showed that this method had strong specificity and a sensitivity of up to 5 pg/μL and suitable for rapid detection of Parastagonospora pseudonodorum.
作者
李金庆
王英超
邵秀玲
季蕾
张静
尹伟力
LI Jin-qing;WANG Ying-chao;SHAO Xiu-ling(Technical Center of Qingdao Customs,Qingdao,Shandong 266109;Technical Center of Yantai Customs,Yantai,Shandong 264000;Linyi Vocational University of Science and Technology,Linyi,Shandong 276000)
出处
《安徽农业科学》
CAS
2024年第18期118-119,132,共3页
Journal of Anhui Agricultural Sciences
基金
国家重点研发计划(2021YFD1400100,2021YFD1400103)
青岛海关科研项目(QK202053,QK202016)。
