基于MiR-21分析左金丸治疗溃疡性结肠炎的作用机制

Analysis of the mechanism of action of Zuojin pill in the treatment of ulcerative colitis based on MiR-21

目的通过观察左金丸对溃疡性结肠炎(UC)小鼠的影响,从miR-21→STAT3/PI3K信号通路研究左金丸治疗溃疡性结肠炎的作用机制。方法将60只BALB/c小鼠随机分为空白组、模型组、美沙拉嗪组(MS,0.3 g/kg)、左金丸组(6 g/kg),用葡聚糖硫酸钠(DSS)构建急性UC小鼠模型,造模第二天开始灌胃给药,持续7 d,观察小鼠一般情况。采用HE染色法观察小鼠结肠组织形态,Western blot法检测小鼠结肠部位的STAT3、PI3K、AKT蛋白表达;运用qrt-PCR法检测小鼠结肠部位的miR-21基因表达。结果左金丸溶液和MS溶液对UC模型小鼠有明显治疗作用,且左金丸组小鼠效果更为显著,通过对STAT3、PI3K、AKt、Mir-21的抑制,减轻对结肠组织的损伤。结论左金丸对UC小鼠免疫调节有效,且miR-21应为左金丸干预UC小鼠的关键环节。

Objective By observing the effect of Zuojin Pill on ulcerative colitis(UC)model mice,the mechanism of action of Zuojin Pill in the treatment of ulcerative colitis was studied from the miR-21→STAT3/PI3K signaling pathway.Methods Sixty BALB/c mice were randomly divided into a blank group,a model group,a mesalazine group(MS,0.3 g/kg),and a Zuojin pill group(6 g/kg),and an acute UC mouse model was constructed with dextran sodium sulfate(DSS),and the drug was administered by gavage starting on the second day of modelling and continuing for 7 days,and the general conditions of the mice were observed.HE staining was used to observe the morphology of mouse colon tissue,and Western blot method was used to detect the expression of STAT3,PI3K and AKt proteins in mouse colon tissue.Quantitative Real-time PCR was used to detect miR-21 gene expression in mouse colon tissue.Results Zuojin pill solution and MS solution had significant therapeutic effects on UC model mice,and the effect was more significant in the Zuojin pill group of mice,reducing the damage to colon tissue by inhibiting STAT3,PI3K,AKt and miR-21.Conclusion Zuojin pill is effective in immunomodulation of UC mice,and miR-21 should be the key link of Zuojin pill intervention in UC mice.