滚针对黄褐斑模型小鼠的作用探讨

Investigation on the function of needle roller on mouse model for melasma

目的探讨滚针对黄褐斑模型小鼠的治疗效果。方法采用完全随机分组法将小鼠分为空白组、模型组、0.20 mm组、0.25 mm组及0.30 mm组,每组各6只。采用黄体酮配合紫外线照射法建立黄褐斑小鼠模型,给予滚针干预治疗后,测定各组皮肤以及肝脏中的丙二醛(MDA)含量、超抗氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性;计算免疫器官指数并观察其在各组小鼠中的变化。结果模型组小鼠的免疫器官指数低于不同长度滚针治疗组和空白组,差异有统计学意义(P<0.05)。模型组小鼠皮肤和肝脏中的SOD、GSH-Px活性水平低于空白组,MDA含量高于空白组,差异有统计学意义(P<0.05)。使用滚针干预后,发现0.20 mm滚针治疗组小鼠皮肤和肝脏的SOD、GSH-Px活性高于模型组,MDA含量低于模型组,差异有统计学意义(P<0.05)。结论滚针治疗可以上调黄褐斑模型小鼠的SOD、GSH-Px活性水平、降低MDA含量,其中,0.20 mm长度滚针的治疗效果较好。

Objective To investigate the therapeutic efficacy of needle roller on mouse model for melasma.Methods A total of 30 mice were selected and divided into the blank group(n=6),the model group(n=6),the 0.20 mm group(n=6),the 0.25 mm group(n=6)and the 0.30 mm group(n=6)according to the complete random grouping method.The mouse model for melasma was established by progesterone combined with ultraviolet irradiation.After treatment of needle roller intervention,the malondialdehyde(MDA)content,superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activity in skin and liver of each group were measured.The immune organ index was calculated and its changes in each group of mice were observed.Results The immune organ index of mice in the model group was lower than that in the needle roller treatment group and the blank group with different lengths,with statistically significant difference(P<0.05).The activity levels of SOD and GSH-Px in the skin and liver of mice in the model group were lower than those in the blank group,and the content of MDA was higher than that in the blank group,with statistically significant differences(P<0.05).After intervention with needle roller,it was found that the activities of SOD and GSHPx in skin and liver of mice treated with 0.20 mm needle roller were higher than those of the model group,and the content of MDA was lower than that of the model group,with statistically significant differences(P<0.05).Conclusion Needle roller therapy can increase the activity levels of SOD,GSH-Px and decrease the content of MDA in mouse model for melasma,and the therapeutic efficacy of 0.20 mm needle roller is better.