无花果原花青素超声辅助提取工艺优化及抗氧化性研究

Ultrasonic-Assisted Extraction Process Optimization and Antioxidant Activity of Proanthocyanidins from Ficus carica L.

以无花果为原料,采用单因素实验结合响应面法对超声辅助提取无花果原花青素(Ficus carica L.Proanthocyanidin,FCPC)工艺进行优化,同时测定DPPH自由基清除率、羟基自由基清除率和总抗氧化能力来综合评价其抗氧化活性。结果表明,FCPC最优提取条件为乙醇体积分数68%,液料比41∶1(mL∶g),提取时间29 min,超声温度47℃,FCPC平均提取量为(15.75±0.28)mg/g,接近模型预测值(15.60 mg/g)。FCPC具有良好的抗氧化能力,对DPPH和OH自由基均具有较强的清除能力,当FCPC质量浓度为0.013 2 mg/mL时,对DPPH和OH自由基的清除率分别可达为94.64%和70.73%。优化的FCPC提取工艺条件稳定可靠,能在实际生产中应用;提取的FCPC具有较强的抗氧化活性,其抗氧化能力大于同质量浓度的维生素C。

Taking Ficus carica L.as raw material,the ultrasonic assisted extraction of Ficus carica L.proanthocyanidins(FCPC) is optimized by single factor combined with response surface methodology.At the same time,the DPPH radical,hydroxyl radical scavenging rate and total antioxidant capacity of FCPC are measured to comprehensively evaluate its antioxidant activity.The results show that the optimal extraction conditions of FCPC are as follows:ethanol volume fraction 68%,liquid-solid ratio 41 to 1(mL to g),extraction time 29 min,ultrasonic temperature 47 ℃,and the average extraction amount of FCPC is(15.75 ± 0.28) mg/g,which is close to the model prediction value(15.60 mg/g).The antioxidant test results show that FCPC have good antioxidant capacity,which have strong scavenging capacity for DPPH radical and OH radical,when the mass concentration of FCPC is 0.0132 mg/mL,the scavenging capacity for DPPH radical and OH radical reachs to 94.64% and 70.73%,respectively.The optimized FCPC extraction process conditions are stable and reliable,which can be applied in practical production;the extracted FCPC has strong antioxidant activity,and its antioxidant capacity is greater than that of vitamin C at the same mass concentration.