响应面法优化枯草芽孢杆菌发酵废渣超声辅助提取蛋白工艺

Response Surface Optimization of Ultrasound Assisted Protein Extraction Process from Bacillus subtilis Fermentation Residue

从发酵废渣中提取蛋白是废弃物综合利用的有效途径之一。本研究在单因素试验的基础上,通过响应面法优化枯草芽孢杆菌发酵废渣的蛋白提取工艺。试验结果表明,在缓冲液pH 9.59、缓冲液体积35 mL(料液比1∶350,g∶mL)、超声温度80℃、超声时间40 min的条件下,可提取蛋白量为(70.42±0.53)mg/g,相比优化前提高了197%。该工艺能为后续相关蛋白的研究及应用提供技术支撑和原料供给。

Protein extraction from fermentation residue is a practical method for waste utilization.Based on a single factor experiment,the response surface methodology is applied to optimize the protein extraction process from Bacillus subtilis fermentation residue.The results indicate that the protein extraction amount significantly increased to(70.42± 0.53) mg/g,a 197% improvement compared to pre-optimization levels,when using buffer pH 9.59,buffer volume of 35 mL(the ratio of solid to liquid is 1 to 350,the units are g and mL,respectively),ultrasound temperature of 80 ℃,and ultrasound time of 40 minutes.This optimized process can offer valuable technical support and raw materials for future research and applications involving related proteins.