元素引发对Na_(2)CO_(3)胁迫下膜荚黄芪种子萌发的影响

The effect of elemental priming on the germinationof membranous Astragalus membranaceus seeds under Na_(2)CO_(3)stress

为研究元素引发对Na_(2)CO_(3)胁迫下膜荚黄芪种子及幼苗生长的影响,以膜荚黄芪种子为研究对象,利用2×10^(-2)mol/L浓度的Na_(2)CO_(3)溶液提供胁迫环境,使用B,Si,Fe,Zn_(4)种元素处理胁迫下的种子,测定了种子的发芽指标、幼苗的生物量、叶绿素含量、渗透调节物质含量和抗氧化酶活力.结果表明:3×10^(-5)mol/L的B元素、6.5×10^(-5)mol/L的Si元素、1.8×10^(-4)mol/L的Fe元素和1.2×10^(-4)mol/L的Zn元素对种子及幼苗的生长发育促进效果显著,可缓解Na_(2)CO_(3)对植物的胁迫作用、提高种子发芽率、增加幼苗生物量的积累、增强幼苗的光合作用、提高幼苗的抗氧化能力.引发浓度较低时,对种子及幼苗生长的促进效果不显著,引发浓度较高时则会抑制种子及幼苗生长.

To investigate the effects of element induced stress on the growth of Astragalus membranaceus seeds and seedlings under Na_(2)CO_(3)stress,Astragalus membranaceus seeds were taken as the research object,and a Na_(2)CO_(3)solution with a concentration of 2×10^(-2)mol/L was used to provide a stress environment.Four elements,B,Si,Fe,and Zn,were used to treat the seeds under stress.The germination indicators,biomass,chlorophyll content,osmoregulatory substance content,and antioxidant enzyme activity of the seeds were measured,providing theoretical reference for the future cultivation and production of Astragalus membranaceus in saline alkali soil.The results showed that 3×10^(-5)mol/L of B element,6.5×10^(-5)mol/L of Si element,1.8×10^(-4)mol/L of Fe element,and 1.2×10^(-4)mol/L of Zn element significantly promoted the growth and development of seeds and seedlings,alleviated the stress of Na_(2)CO_(3)on plants,increased seed germination rate,increased biomass accumulation of seedlings,enhanced photosynthesis of seedlings,and improved antioxidant capacity of seedlings.When the priming concentration is low,the promotion effect on the growth of seeds and seedlings is not significant,while when the priming concentration is high,it will inhibit the growth of seeds and seedlings.