佛手香豆素类成分分析

Analysis of Coumarins in Citrus medica

目的:研究佛手枝、叶香豆素类化学成分。方法:应用溶剂浸取法、萃取法、大孔树脂柱色谱、硅胶色谱、凝胶色谱、薄层色谱以及制备型高效液相色谱等方法对佛手枝和叶的化学成分进行了分离纯化;并通过理化性质及1H-NMR、13C-NMR等波谱技术对化合物的结构进行鉴定。结果:从佛手枝、叶中共分离了8个香豆素类化合物,分别为东莨菪内酯(1)、水合氧化前胡素(2)、2'-甲氧基氧化前水胡素(3)、5,7-二甲氧基香豆素(4)、佛手柑内酯(5)、5-羟基-7-甲氧基香豆素(6)、7-羟基-5-甲氧基香豆素(7)、7-[(2E,6R)-6,7-二羟基-3,7-二甲基-2-辛基-1-基]氧基-香豆素(8)。结论:分离得到的8个香豆素类化合物,其中1、2、3号化合物为首次从佛手叶中分离出来,6、7、8号化合物为首次从佛手枝中分离出来,可为佛手的香豆素类成分分析提供一定的科学依据。

Objective:To study the the chemical constituents of coumarins from the branches and leaves in Citrus medica L.var.sarcodactylis Swingle.Methods:The separation and purification of the chemical components of Citrus medica branches and leaves were carried out by solvent extraction,extraction,macroporous resin column chromatography,silica gel chromatography,gel chromatography,thin-layer chromatography,and preparative high-performance liquid chromatography.The structures of the compounds were characterized by physicochemical properties,1H-NMR and 13C-NMR spectroscopy.Results:Eight coumarins were isolated from the branches and leaves of Citrus medica.Their structures were identified as Scopoletin(1),Oxypeucedanin hydrate(2),2'-Methoxyproarlin hydrate(3),5,7-Dimethoxycoumarin(4),Bergapten(5),5-Hydroxy-7-methoxycoumarin(6),7-Hydroxy-5-methoxycoumarin(7),7-[[(2E,6R)-6,7-Dihydroxy-3,7-dimethyl-2-octen-1-yl]oxy]-2H-1-benzopyran-2-one(8).Conclusion:Among the eight coumarins,compounds 1,2 and 3 were isolated from the leaves of Citrus medica for the first time,and compounds 6,7 and 8 were isolated from the branches of Citrus medica for the first time,it can provide a scientific basis for the analysis of coumarins in Citrus medica.