基于指纹图谱结合化学模式识别法评价不同产地蒲桃叶药材质量

Quality evaluation of Syzygium jambos(L.)Alston leaves from different origins based on fingerprint combined with chemical pattern recognition

建立蒲桃叶HPLC指纹图谱并指认其中2种成分,结合主成分和聚类分析对不同产地蒲桃叶综合品质进行分析,为其质量评价提供理论和数据基础。采用Sonoma C_(18)(2)(4.6 mm×250 mm,5μm)色谱柱,0.1%甲酸水-甲醇为流动相,梯度洗脱,检测波长290 nm,流速1.0 mL/min,进样量10μL,柱温30℃,采用《中药色谱指纹图谱相似度评价系统(2012版)》进行相似度评价,确定共有峰。采用SPSS 22.0统计软件进行聚类分析、主成分分析,建立不同产地蒲桃叶的综合评价表。10批蒲桃叶HPLC指纹图谱标定12个共有峰,指认了2种化学成分,1号峰为没食子酸,4号峰为对羟基苯甲酸。10批蒲桃叶可聚为三类,茂名产蒲桃叶聚为一类,肇庆蒲桃叶聚为一类,其余地区产蒲桃叶聚为一类。主成分、因子分析表明肇庆蒲桃叶质量总体优于其他产地,1、2、4、5、6、12共有峰可作为蒲桃叶质量评价主要成分。10批样品相似度为0.591~0.994,表明不同批次蒲桃叶样品存在差异。所建HPLC指纹图谱和含量测定方法简便、稳定性好,结合化学模式可为蒲桃叶药材质量控制与评价提供参考。

This study aims to study comprehensively the quality of Syzygium jambos(L.)Alston leaves from different populations based on the methods of HPLC fingerprint and chemical pattern recognition,and provide reference for its quality evaluation.The Sonoma C_(18)(2)column(4.6 mm×250 mm,5μm)was used for the analysis,with a 0.1%formic acid water-methanol solution serving as the mobile phase,detection wavelength of 290 nm,flow rate of 1.0 mL/min,injection volume of 10μL,and a column temperature of 30℃.The similarity evaluation was performed using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)to determine the common peak.SPSS 22.0 statistical software was used for cluster analysis and principal component analysis to establish a comprehensive evaluation table of S.jambos leaves from different origins.The HPLC fingerprint of S.jambos leaves was established for the first time,the similarity was 0.591-0.994,and 12 common peaks were calibrated.Through comparison with the reference substance,two components were identified:gallic acid and p-hydroxybenzoic acid.The ten batches of S.jambos leaves were divided into three groups during chemical pattem recognition analysis,the first group was primarily from Maoming,the second group was primarily from Zhaoqing,and thired group was primarily from the others origins.Principal component analysis and factor analysis showed that the quality of S.jambos leaves from Zhaoqing was generally better than that in other origins.The common peaks of 1,2,4,5,6 and 12 could be used as the main components for the quality evaluation of S.jambos leaves.The method of combining HPLC fingerprint and chemical pattern recognition can not only provide a reference for the comprehensive evaluation of the quality control of S.jambos leaves,but also provide a certain scientific basis for the quality evaluation of its germplasm resources.