油橄榄叶提取物对HaCaT细胞氧化损伤的保护作用及抑菌活性

Protective effect of Olea europaea leaves extract against oxidative damage in HaCaT cells and its antibacterial activity

为研究油橄榄叶提取物对人永生化角质形成细胞(HaCaT)氧化损伤的保护作用及抑菌作用,采用肌红蛋白保护率评价油橄榄叶提取物对不同种类自由基的胞外抗氧化能力,建立HaCaT细胞的氧化损伤和炎症模型,评价油橄榄叶提取物对细胞氧化损伤的保护作用,并进一步考察油橄榄叶提取物对常见病原菌的抑制作用。结果表明油橄榄叶提取物对羟基自由基、次氯酸根和过氧自由基均具有抗氧化作用,其中对羟基自由基和过氧自由基的抗氧化效果较好。在细胞水平上,油橄榄叶提取物能够显著降低HaCaT的胞内自由基,可以有效改善HaCaT细胞的氧化应激状态。同时,0.156~0.625μg/mL的油橄榄叶提取物能够有效降低炎症细胞中的白细胞介素6(interleukin-6,IL-6)和白细胞介素1β(interleukin-1β,IL-1β)因子水平,0.156~0.312μg/mL的提取物能够降低肿瘤坏死因子(tumor necrosis factor-α,TNF-α)水平。此外,油橄榄叶提取物能够有效抑制金黄色葡萄球菌,而对大肠杆菌和铜绿假单胞菌敏感度低。综上,油橄榄叶提取物对HaCaT细胞的氧化损伤具有一定的保护作用,且具有抗炎效果和抑菌活性。

To investigate the protective effect of Olea europaea leaves extract against oxidative damage in HaCaT cells and its antibacterial activity,the myoglobin protective ratio was used to evaluate the extracellular antioxidant capacity of O.europaea leaves extract against different types of free radicals,the oxidative damage and inflammation model of HaCaT cells was established to evaluate the protective effect of the extract against oxidative damage to the cells,and the inhibitory effect of O.europaea leaves extract on the common pathogenic bacteria was further investigated.The results showed that the O.europaea leaves extract possessed antioxidant effects on hydroxyl radicals,hypochlorite and peroxyl radicals,among which the antioxidant effects on hydroxyl and peroxyl radicals were better.At the cellular level,the extract was able to significantly reduce the intracellular free radicals of HaCaT,which could effectively improve the oxidative stress status of HaCaT cells.Meanwhile,0.156-0.625μg/mL of O.europaea leaves extract was effective in reducing IL-6,IL-1βlevels in inflammatory cells,and 0.156-0.312μg/mL of the extract was able to reduce TNF-αlevels.In addition,O.europaea leaves extract was able to effectively inhibit Staphylococcus aureus with low sensitivity to Escherichia coli and Pseudomonas aeruginosa.In conclusion,O.europaea leaves extract has a certain protective effect against oxidative damage in HaCaT cells,and has anti-inflammatory and antibacterial activities.