年龄相关性白内障患者血清miR-26a和miR-26b表达水平与晶状体混浊的相关性

Correlation of Serum miR-26a and miR-26b Expression Levels with Lens Opacity in Patients with Age-Related Cataract

目的 研究年龄相关性白内障患者血清miR-26a和miR-26b表达水平与晶状体混浊的相关性。方法 选择2020年2月至2023年2月期间在我院检查出的年龄相关性白内障患者70例作为研究组,同期在我院进行常规体检的健康志愿者68为对照组,采用RT-PCR检测血清中的miR-26a和miR-26b的表达水平。结果 研究组血清miR-26a及miR-26b的表达水平低于对照组(P<0.05),miR-26a、miR-26b表达与LOCSⅢ分级N、C、P评分呈负相关(P<0.05),血清miR-26a和miR-26b表达量对晶状体浑浊具有一定预测价值。结论 年龄相关性白内障患者血清miR-26a、miR-26b表达水平均降低,miR-26a与miR-26b低水平表达与白内障晶状体浑浊程度有关。

Objective To discuss the correlation of serum miR-26a and miR-26b expression levels with lens opacity in patients with age-related cataract.Methods Seventy patients with age-related cataract in our hospital from February 2020 to February 2023 were enrolled as study group, meantime, another 68 healthy individuals underwent physical examination in our hospital during the same period were set as control group.The reverse transcription polymerase chain reaction(RT-PCR)was used to detect the expression levels of miR-26a and miR-26b in serum.Results The expression levels of serum miR-26a and miR-26b in study group were lower than those in control group(P<0.05).The expression of miR-26a and miR-26b was negatively correlated with the scores of nuclear(N),cortical(C),and posterior subcapsular(P)cataract in Lens Opacities Classification System II(LOCS),with statistical significance(P<0.05).The expression levels of serum miR-26a and miR-26b had certain predictive value for lens opacity.Conclusion Serum expression levels of miR-26a and miR-26b are down-regulated in patients with age-related cataract, and the low expression of two indicators are closely related to the lens opacity.