加热卷烟烟气对肺表面活性物质单层膜的影响

The effect of heated cigarette smoke on the monolayer of pulmonary surfactant

目的 探究加热卷烟烟气入肺后,其对肺表面活性物质(Pulmonary surfactant,PS)单层膜的影响,揭示其可能会对肺功能和健康造成损害。方法 本研究采用加热卷烟烟气制备水溶液和从猪肺中提取天然PS作为实验材料,测定加热卷烟烟气暴露前后PS表面张力、起泡性能和相行为等界面化学性质的变化。结果 加热卷烟烟气会对PS中的磷脂和蛋白组分产生吸附作用(P<0.05)导致磷脂和蛋白含量下降,进而使表面张力升高和起泡性能下降,在最大暴露量时磷脂和蛋白含量从46.86 mg/L和5.09 mg/L降至28.16 mg/L和3.52 mg/L,表面张力从27.37 mN/m升至37.76 mN/m,起泡性能从48.56 ml降至34.57 ml。由布鲁斯特角显微镜观察得到,烟气组分(颗粒物和有机物)还会嵌入和积累在PS薄膜上,导致PS膜的π-A等温线外扩。结论 加热卷烟烟气的暴露可能会影响PS在肺泡内的正常功能,从而对肺部健康造成潜在的危害。

Objective To explore the effect of heated cigarette smoke on pulmonary surfactant(PS)monolayer after it enters the lung,and to reveal that it may cause damage to lung function and health.Methods In this study,aqueous solution prepared by heating cigarette smoke and natural PS extracted from pig lungs were used as experimental materials to determine the changes of interfacial chemical properties such as surface tension,foaming properties and phase behavior of PS before and after exposure to heated cigarette smoke.Results Heating cigarette smoke will adsorb phospholipids and protein components in PS(P<0.05),resulting in a decrease in phospholipids and protein content,which in turn increases surface tension and decreases foaming performance.At the maximum exposure,phospholipids and protein content decreased from 46.86 mg/L and 5.09 mg/L to 28.16 mg/L and 3.52 mg/L,surface tension increased from 27.37 mN/m to 37.76 mN/m,and foaming performance decreased from 48.56 ml to 34.57 ml.It was observed by the Brewster angle microscope that the flue gas components(particulate matter and organic matter)were also embedded and accumulated on the PS film,resulting in the outward expansion of theπ-A isotherm of the PS film.Conclusion Exposure to heated cigarette smoke may affect the normal function of PS in the alveoli,which may pose a potential hazard to lung health.