PPP2R1A对Hela细胞系中调节癌症相关基因表达的影响

Effect of PPP2R1A on regulating the expression of cancer-related genes in Hela cells

目的分析PPP2R1A对Hela细胞系中调节癌症相关基因表达的影响。方法以未干预的Hela细胞系作为对照组,以克隆并过表达PPP2R1A的Hela细胞系作为过表达组。通过RNA测序分析对照组和过表达组细胞中PPP2R1A的表达和选择性剪接(AS)。通过实时荧光定量聚合酶链反应(RT-qPCR)实验验证PPP2R1A参与调控的肿瘤转移相关基因和选择性剪接基因(ASGs)。结果与对照组比较,过表达PPP2R1A可显著抑制Hela细胞的增殖。PPP2R1A调控细胞黏附、病毒应答、细胞生长调节等数百个基因的表达水平,同时还调节参与细胞周期、连接黏附、子宫内膜癌和RNA转运基因的AS。结论PPP2R1A可能通过AS调控潜在转录来调控肿瘤的进展。

Objective To investigate effects of PPP2R1A on regulating the expression of cancer-related genes in Hela cell lines.Methods The Hela cell lines without intervention was used as the control group,and the Hela cell line with cloned and overexpressed PPP2R1A gene was used as the overexpression group.The expression and alternative splicing(AS)of PPP2R1A gene in the control and overexpression groups were analyzed by RNA sequencing.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to verify the involvement of PPP2R1A in tumor metastasis-related genes and alternative splicing genes(ASGs).Results Compared with the control group,PPP2R1A overexpression significantly inhibited the proliferation of Hela cells.PPP2R1A regulates the expression levels of hundreds of genes,including cell adhesion,viral response,cell growth regulation,and also regulates AS of genes involved in cell cycle,ligand adhesion,endometrial cancer,and RNA transport.Conclusion PPP2R1A may regulate tumor progression through AS regulation of latent transcription.