结直肠癌根治术后癌组织中CK20、P53、MSH6、Ki67的表达及其病理意义

Expression and pathological significance of CK20,P53,MSH6 and Ki67 in cancer tissues after radical surgery for colorectal cancer

目的探讨结直肠癌根治术后癌组织中细胞角蛋白20(CK20)、p53肿瘤蛋白(P53)、错配修复酶6(MSH6)及细胞增殖核抗原Ki67(Ki67)表达及病理意义。方法回顾性分析2022年1月至2024年5月到本院就诊并接受结直肠癌根治术的93例患者的临床资料。采用免疫组化方法检测患者癌组织及癌旁组织中CK20、P53、MSH6及Ki67的表达情况,并结合患者基线资料,对各指标表达状况与临床特征之间的关系进行分析。结果癌组织中CK20、P53及Ki67阳性占比高于癌旁组织,MSH6阴性占比高于癌旁组织(P<0.05)。性别、年龄、病灶位置、是否有淋巴结转移、肿瘤直径、浸润深度及分化程度与CK20表达状况之间关系不显著(P>0.05)。CK20阳性表达与临床分期相关(P<0.05)。性别、年龄、病灶位置及肿瘤直径与P53表达状况之间关系不显著(P>0.05)。P53阳性表达与是否有淋巴结转移、浸润深度、分化程度及临床分期相关(P<0.05)。性别、病灶位置、是否有淋巴结转移、肿瘤直径、浸润深度、分化程度及临床分期与MSH6表达状况之间关系不显著(P>0.05)。MSH6阴性表达与年龄相关(P<0.05)。性别、年龄、病灶位置及分化程度与Ki67表达状况之间关系不显著(P>0.05)。Ki67阳性表达与是否有淋巴结转移、肿瘤直径、浸润深度及临床分期相关(P<0.05)。结论CK20、P53、MSH6及Ki67表达状况与结直肠癌根治术后患者病理特征密切相关,可对结直肠癌进行评估,并对治疗提供指导意义。

Objective To investigate the expression and pathological significance of cytokeratin 20(CK20),p53 tumor protein(P53),mis-match repair enzyme 6(MSH6)and cell proliferation nuclear antigen Ki67(Ki67)in cancer tissues after radical surgery for colorectal cancer.Methods Clinical data of 93 patients admitted to our hospital from January 2022 to May 2024 and underwent radical surgery for colorectal cancer were retrospectively analyzed.Immunohistochemical methods were used to detect the expression of CK20,P53,MSH6 and Ki67 in the cancer tissues and paracance tissues of the patients,and the relationship between the expression status of the indexes and the clinical characteristics was analyzed in combination with the baseline data of the patients.Results The percentages of CK20,P53 and Ki67 positivity were higher in cancer tissues than those in paracancer tissues,and the percentage of MSH6 negativity was higher than that in paracancer tissues(P<0.05).The relationship between gen-der,age,location of the lesion,presence of lymph node metastasis,tumor diameter,depth of infiltration and degree of differentiation and CK20 expres-sion status was not significant(P>0.05).CK20 positive expression was correlated with clinical stage(P<0.05).The relationship between gender,age,le-sion location and tumor diameter and P53 expression status was not significant(P>0.05).P53-positive expression was correlated with the presence of lymph node metastasis,depth of infiltration,degree of differentiation and clinical stage(P<0.05).The relationship between gender,lesion location,presence of lymph node metastasis,tumor diameter,depth of infiltration,degree of differentiation and clinical stage and MSH6 expression status was not significant(P>0.05).MSH6 negative expression was correlated with age(P<0.05).There was no significant relationship between gender,age,loca-tion of lesions and degree of differentiation and Ki67 expression status(P>0.05).Ki67-positive expression was correlated with the presence of lymph node metastasis,tumor diameter,depth of infiltration,and clinical stage(P<0.05).Conclusion The expression status of CK20,P53,MSH6 and Ki67 is closely related to the pathological characteristics of patients after radical surgery for colorectal cancer,which can be evaluated and provide guidance for the treatment of colorectal cancer.