山药块茎生长膨大相关基因的筛选与分析

Screening and Analysis of Genes Related to Growth and Expansion of Yam(Dioscorea alata L.)Tubers

为筛选山药块茎生长膨大相关基因,采用生物信息学方法,对山药块茎发育前期和中期构建的不同发育阶段抑制性消减杂交(suppression subtractive hybridization,SSH)文库进行分析。结果表明:山药块茎发育前期与中期差异表达基因(differential expression genes,DEGs)序列主要涉及九大类,其中发育调控占比最大(27.1%),其他依次为细胞组织结构(14.0%)、物质代谢(11.2%)、生物合成(4.7%)等。初步筛选出泛素-连接酶体(F-box associated,F-box)、碳酸酐酶(carbonic anhydrase,CAs)、核苷二磷酸激酶(nucleoside diphosphate kinase,NDPK)、氨基酸透性酶(amino acid permease)、糖基转移酶(glycosyl transferase)、α-淀粉酶(α-amylase,AMY)等候选基因,它们可能与细胞增殖、细胞伸长、激素代谢、酸碱环境调节及功能物质代谢物等功能有关,参与调控山药块茎的生长膨大。

To screen the genes related to the growth and expansion of yam tubers,bioinformatics methods were used to analyzed the suppression subtractive hybridization(SSH)libraries at different developmental stages of yam tubers in the early and middle stages.The results showed that the differentially expressed genes(DEGs)sequences in the early and middle stages of the development of yam tubers mainly involved nine categories,of which developmental regulation accounts for the largest proportion(27.1%),followed by cellular tissue structure(14.0%),material metabolism(11.2%),and biosynthesis(4.7%).Preliminary screening has identified some genes,including F-box associated,carbonic anhydrase(CAs),nucleoside diphosphate kinase(NDPK),amino acid permease,glycosyl transferase,and alpha amylase.These genes may be related to cell proliferation,elongation,hormone metabolism,acid-base environment regulation,and functional metabolite regulation,and are involved in regulating tuber growth and expansion in yam.