高危型HPV感染临床检验中实时荧光定量PCR检测法的作用

Role of quantitative real-time PCR detection in clinical testing of high-risk HPV infection

目的分析高危型人乳头瘤病毒(HPV)感染临床检验中实时荧光定量聚合酶链式反应(PCR)检测法的作用。方法选取80例疑似高危型HPV感染患者,均接受第二代杂交式捕获法(HCⅡ)、实时荧光定量PCR检测,以病理检查结果为金标准,分析实时荧光定量PCR检测价值。结果实时荧光定量PCR检验准确率97.50%、敏感度98.51%、特异度92.31%,均显著高于HCⅡ的78.75%、88.06%、38.46%(P<0.05);实时荧光定量PCR诊断检出率CINⅠ100%、慢性宫颈炎92.31%,均显著高于HCⅡ的88.10%、46.15%(P<0.05)。结论应用实时荧光定量PCR检测法可明显提高高危型HPV感染患者的临床检验准确性,提高治疗方法的针对性。

Objective To analyze the role of real-time fluorescence quantitative polymerase chain reaction(PCR)detection method in clinical testing of high-risk human papillomavirus(HPV)infection.Methods 80 suspected high-risk HPV infection patients were selected,all of whom underwent second-generation hybrid capture method(HCⅡ)and real-time fluorescence quantitative PCR detection.The pathological examination results were used as the gold standard to analyze the value of real-time fluorescence quantitative PCR detection.Results The accuracy,sensitivity,and specificity of real-time fluorescence quantitative PCR were 97.50%,98.51%,and 92.31%,respectively,which were significantly higher than the 78.75%,88.06%,and 38.46%of HCⅡ(P<0.05);The detection rates of CINⅠ100%and chronic cervicitis 92.31%in real-time fluorescence quantitative PCR diagnosis were significantly higher than those of HCⅡ88.10%and 46.15%(P<0.05).Conclusion The application of real-time fluorescence quantitative PCR detection can significantly improve the clinical testing accuracy of high-risk HPV infection patients and enhance the targeted treatment methods.