IL-18、IL-18BP、IL-18R在特应性皮炎患者外周血B淋巴细胞及单核细胞中的表达

Expression of IL-18,IL-18BP,and IL-18R in peripheral blood B lymphocytes and monocytes of patients with atopic dermatitis

目的探讨特应性皮炎(AD)患者外周血B淋巴细胞及单核细胞中白细胞介素(IL)-18、IL-18BP和IL-18R的表达。方法收集28例AD患者和21例健康对照者外周静脉血,分别用大籽蒿过敏原提取物、尘螨过敏原提取物或梧桐花粉过敏原提取物刺激,采用流式细胞术检测B淋巴细胞及单核细胞中IL-18^(+)、IL-18BP^(+)和IL-18R^(+)的表达。结果与健康对照者比较,AD患者静息状态下B淋巴细胞群中IL-18^(+)、IL-18BP^(+)、IL-18R^(+)细胞比例分别升高2.01、10.35、20.85倍,单核细胞群中IL-18^(+)和IL-18BP^(+)细胞比例分别升高5.51和41.88倍,IL-18R^(+)细胞比例无明显变化。结论B淋巴细胞及单核细胞中IL-18、IL-18BP和IL-18R可能在AD中起重要作用,可能是治疗AD的潜在靶点。

Objective To investigate the expression of IL-18,IL-18BP,and IL-18R in the peripheral blood B lymphocytes and monocytes of patients with atopic dermatitis(AD).Methods Peripheral venous blood was collected from 28 patients with AD and 21 healthy controls,and stimulated with Artemisia sieversiana wild allergen extract,house dust mite allergen extract,or Platanus pollen allergen extract.The expression of IL-18^(+),IL-18BP^(+),and IL-18R^(+)in B lymphocytes and monocytes was measured using flow cytometry.Results Compared with the healthy control group,the proportions of IL-18^(+),IL-18BP^(+),and IL-18R^(+)cells in the B lymphocyte group of patients with AD at rest increased 2.01-,10.35-,and 20.85-fold,respectively.The proportions of IL-18^(+)and IL-18BP^(+)cells in monocytes increased 5.51-and 41.88-fold,respectively,whereas the proportion of IL-18R^(+)cells did not differ significantly between the groups.Conclusion IL-18,IL-18BP,and IL-18R in B lymphocytes and monocytes may play an important role in AD.IL-18,IL-18BP,and IL-18R may be potential targets for the treatment of AD.