基于葡萄糖诱导的秀丽隐杆线虫模型探讨黄芪抗氧化的作用及机制

Astragalus membranaceus based on a glucose-induced Caenorhabditis elegans model

本研究考察黄芪对高糖诱导的秀丽隐杆线虫的作用并探讨其作用机制。采用UPLC-MS法鉴定黄芪的成分。以秀丽隐杆线虫为模式生物,建立高糖模型,测定黄芪对线虫的体长、头尾摆动、咽泵运动能力、活性氧(reactiveoxygenspecies,ROS)的影响;运用实时荧光定量聚合酶链式反应(polymerasechainreaction,PCR)检测黄芪对BZIP结构域蛋白1(protein skinhead-1,SKN-1)信号通路相关基因mRNA表达的影响。结果显示,与正常组相比,高糖状态下线虫体长、头尾摆动、咽泵频率表达均显著降低,体内ROS含量显著增加;黄芪给药后体长、头尾摆动、咽泵频率表达均显著增加,ROS含量显著降低(P<0.01)。与正常组相比,高糖状态下秀丽隐杆线虫中SKN-1、超氧化物歧化酶3(superoxidedismutas-3,SOD-3)、抗氧化基因谷胱甘肽S-转移酶4(glutathioneS-transferase4,GST-4)、谷胱甘肽S-转移酶7(glutathioneS-transferase7,GST-7)的表达均显著降低;黄芪给药后SKN-1、SOD-3、GST-4、GST-7表达均显著增加(P<0.01)。本研究表明,在秀丽隐杆线虫内,黄芪通过调控SKN-1信号通路改善高糖引起的氧化应激反应。

The objective of this study was to observe the effect of Astragalus membranaceus on high sugar-induced Caenorhabditis elegans,and to explore its mechanism of action.UPLC-MS method was used to identify the components of Astragalus membranaceus.A high glucose model was established by using Caenorhabditis elegans as a model organism,and the effects of Astragalus membranaceus on body length,body bending,swallowing frequency,and reactive oxygen species(ROS)of the nematode were determined;the effects of Astragalus membranaceus on the expression of mRNA of genes related to the protein skinhead-1(SKN-1)signaling pathway were examined by using the real-time fluorescence quantitative polymerase chain reaction(PCR).The results showed that compared with the normal group,the nematode body length,body bending,and swallowing frequency expression were significantly reduced and the ROS content in the body was significantly increased in the high glucose state;after the administration of Astragalus membranaceus,the body length,body bending,and swallowing frequency expression were significantly increased,and the ROS content was significantly reduced(P<0.01).Compared with the normal group,SKN-1,superoxide dismutas-3(SOD-3),glutathione S-transferase 4(GST-4),and glutathione S-transferase 7(GST-7)expression were significantly decreased in Caenorhabditis elegans in the high glucose condition;SKN-1,SOD-3,GST-4,and GST-7 expression were significantly increased after administration of Astragalus membranaceus(P<0.01).In the present study,we demonstrated that Astragalus membranaceus has an effect on high glucose-induced Caenorhabditis elegans nematodes,and its mechanism of action may be through the modulation of the SKN-1 signaling pathwaym in order to ameliorate the oxidative stress response induced by high glucose.