基于UPLC-MS/MS和网络药理学研究复方板蓝根颗粒治疗流行性乙型脑炎、肝炎及腮腺炎的作用机制

Study on mechanism of compound Banlangen Granules for epidemic encephalitis B,hepatitis and parotitis based on UPLC-MS/MS and network pharmacology

目的阐明复方板蓝根颗粒中的血中移行成分,探究其治疗流行性乙型脑炎、肝炎、腮腺炎的作用机制。方法采用超高效液相色谱-质谱(UPLC-MS/MS)技术对复方板蓝根颗粒中的血中移行成分进行分析,并结合网络药理学分析复方板蓝根颗粒治疗流行性乙型脑炎、肝炎、腮腺炎中起调控作用的靶点及通路。结果共鉴定出9个入血成分,其中8个是原型成分,分别为蔗糖、邻氨基苯甲酸、尿苷、腺苷、鸟苷、表告依春、吲哚-3-乙腈-2-S-β-D-吡喃葡萄糖苷、水杨酸。通过网络药理学分析得到复方板蓝根颗粒成分可能通过调控脂质与动脉粥样硬化、胰岛素抵抗、IL-17等信号通路治疗乙型脑炎、肝炎、腮腺炎。结论复方板蓝根颗粒的9个入血成分可能通过调控脂质与动脉粥样硬化、胰岛素抵抗、IL-17等信号通路治疗乙型脑炎、肝炎、腮腺炎。

Objective To clarify the transitional components in the blood of compound Banlangen Granules;To explore the mechanism of drugs in the treatment of epidemic encephalitis B,hepatitis and parotitis.Methods The transitional components in blood of compound Banlangen Granules were analyzed by ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS/MS).The regulatory targets and pathways of compound Banlangen Granules in the treatment of epidemic encephalitis B,hepatitis and parotitis were analyzed based on UPLC-MS/MS and network pharmacology.Results A total of 9 blood components were identified,of which 8 were prototype components,including sucrose,o-aminobenzoic acid,uridine,adenosine,guanosine,indole-3-acetonitrile-2 murine-S-β-D-glucopyranoside and salicylic acid.Through network pharmacological analysis,it was concluded that compound Banlangen Granules may treat epidemic encephalitis B,hepatitis and parotitis by regulating lipid and atherosclerosis,insulin resistance,IL-17 and other signal pathways.Conclusion The 9 blood components of compound Banlangen Granules may treat epidemic encephalitis B,hepatitis and parotitis by regulating lipid and atherosclerosis,insulin resistance,IL-17 and other signal pathways.