复方石韦散对大鼠草酸钙晶体形成及滞留的影响

Effects of Compound Shiwei Powder on Calcium Oxalate Crystal Formation and Retention in Rats

目的观察复方石韦散对草酸钙结石大鼠晶体形成及滞留的影响。方法50只SD大鼠随机分为5组,分别为正常对照组(A)、模型组(B),中药组:复方石韦散低(C1)、中(C2)、高(C3)剂量组,采用乙二醇和氯化铵自由饮水1周建造草酸钙晶体大鼠模型,中药组造模同时予以对应中药灌胃,实验第6天收集24 h尿液并检测尿酸、尿钙,7天后处死大鼠,HE染色观察肾组织草酸钙晶体,检测肾组织超氧化物歧化酶(SOD)含量,免疫组化法检测骨桥蛋白(OPN)及损伤因子TIM-1的表达情况。结果成功建立草酸钙晶体大鼠模型,与B组相比,C1、2、3组24 h尿草酸、尿钙随中药浓度升高而下降(P<0.05);结晶HE染色程度评分上,C1、2、3组较B组评分明显下降并有统计学差异(P<0.05),其中C3组评分最低;与B组相比,C各组肾组织SOD活性表达明显升高(P<0.05);与B组相比,C组肾组织OPN、TIM-1表达明显下降(P<0.01)。结论复方石韦散能有效抑制草酸钙结晶形成及减少滞留,其机制可能与通过降低24 h尿草酸、尿钙排泄量,增强抗氧化应激,抑制OPN、TIM-1的表达有关。

Objective To observe the effects of Compound Shiwei Powder on the formation and retention of calcium oxalate crystals in rats.Methods Fifty SD rats were randomized to five groups:normal control(A),model(B),and low-,medium-,and high-dose Compound Shiwei Powder groups(C1,C2,and C3,respectively).The rat model of calcium oxalate crystals was established by free drinking of the water containing ethylene glycol and ammonium chloride for one week.The C1,C2,and C3 groups were administrated with the corresponding agents at the time of modeling.On day 6 of the experiment,24-h urine was collected for the measurement of uric acid and urinary calcium levels.The rats were sacrificed after 7 days.The formation and retention of calcium oxalate crystals in the renal tissue were observed by hematoxylin-eosin staining.The activity of superoxide dismutase(SOD)in the renal tissue was measured,and the expression of osteopontin(OPN)and T-cell immunoglobulin and mucin domain 1(TIM-1)was detected by immunohistochemistry.Results The rat model of calcium oxalate crystals was successfully established.Compared with group B,groups C1,C2,and C3 lowered the levels of 24-h urinary oxalic acid and urinary calcium in a concentrationdependent manner(P<0.05),decreased the crystallization score(P<0.05,being the lowest in the C3 group),increased the activity of SOD(P<0.05),and down-regulated the expression of OPN and TIM-1 in the renal tissue(P<0.01).Conclusion Compound Shiwei Powder can effectively inhibit the formation and retention of calcium oxalate crystals by reducing 24-h uric acid and urinary calcium excretion,enhancing responses to oxidant stress,and inhibiting the expression of OPN and TIM-1.