幼年期双酚 F 暴露对小鼠空间工作记忆的影响

Effects of juvenile bisphenol F exposure on spatial working memory of mice

双酚F(BPF)作为双酚A(BPA)的主要替代物,对大脑学习记忆的影响,特别是空间工作记忆的研究相对较少。文章以幼年期(21~49 d)为时间窗口,通过不同的行为学范式及免疫印迹实验研究BPF暴露对小鼠空间工作记忆的影响。研究发现,1 mg/(kg·d)BPF暴露不会影响小鼠的体质量、饮水量等生理状态和小鼠基本的运动能力,但对小鼠的空间工作记忆能力有损害。在T迷宫40 s测试中,BPF组的正确率显著低于对照组小鼠;前额叶皮层(prefrontal cortex,PFC)谷氨酸受体蛋白中GluR2表达量显著低于对照组。结果表明,幼年期BPF暴露损害小鼠的空间工作记忆能力可能与兴奋性蛋白受体亚基GluR2表达量降低有关。该研究将为BPF引起的神经系统损伤提供了有价值的信息,并在工业生产中为BPA替代物的选择提供了理论依据。

There are relatively few studies on the effects of bisphenol F(BPF),the main substitute for bisphenol A(BPA),on brain learning and memory,especially spatial working memory.In this paper,juvenile(21-49 d)was used as a time window to study the effects of BPF exposure on spatial working memory of mice through different behavioral paradigms and immunoblotting experiments.The study found that 1 mg/(kg·d)BPF exposure did not affect the physiological status of mice such as body weight and water intake,and did not affect the basic exercise ability of mice,but it did damage to the spatial working memory ability of mice.In the T-maze 40 s test,the correct rate of the BPF group was significantly lower than that of the control group;in addition,the expression of GluR2 in the prefrontal cortex(PFC)glutamate receptor protein was significantly lower than that of the control group.These results suggest that juvenile BPF exposure impairs the spatial working memory ability of mice,which may be related to the decreased expression of the excited receptor subunit GluR2.This study will provide valuable information on BPF-induced neurological damage and provide a rationale for the selection of BPA substitutes in industrial production.