^(137)Cs-γ射线辐射对大花威灵仙愈伤生长及分化的影响

Effects of^(137)Cs-γ Ray Radiation on Callus Growth and Differentiation of Clematis courtoisii

以大花威灵仙(Clematis courtoisii)的胚根为试材,诱导出胚性愈伤后,采用激素配比和辐射计量分析方法,研究了激素配比和辐射计量对愈伤组织分化的影响,以期为大威灵仙植物组织培养和诱变种质创新相关研究提供参考依据。结果表明:6-BA 2 mg·L^(-1)+NAA 0.05 mg·L^(-1)是诱导愈伤分化的最佳培养基;使用不同剂量(0、30、60、90、120、150、180 Gy)的^(137)Cs-γ射线进行辐射处理,发现60 Gy辐射剂量对愈伤分化率影响最小,120 Gy为大花威灵仙的半致死剂量。^(137)Cs-γ辐射大花威灵仙愈伤组织的适宜剂量范围是60~90 Gy。

Taking the embryonic roots of Clematis courtoisii as the experimental material,embryogenic callus was induced.Hormone ratio and radiometric analysis methods were used to study the effects of hormone ratio and radiometric analysis on callus differentiation,in order to provide reference for the research on tissue culture and mutation germplasm innovation of Clematis courtoisii.The results showed that 6-BA 2 mg·L^(-1)+NAA 0.05 mg·L^(-1)was the optimal medium for inducing callus differentiation.By using different doses of^(137)Cs-γrays(0,30,60,90,120,150,180 Gy)for radiation treatment,the radiation dose of 60 Gy had the least impact on callus differentiation rate,and 120 Gy was the semi lethal dose of Clematis courtoisii.The appropriate dose range for^(137)Cs-γradiation in the callus tissue of Clematis courtoisii was 60-90 Gy.