甘南州野生羊肚菌菌种分离及母种培养基筛选试验

Experimental on the Isolation of Wild Morchella Fungi in Gannan Prefecture and the Screening of Mother Culture Medium

以采自甘南州境内(舟曲县、临潭县、迭部县)的野生羊肚菌子实体为试材,采用组织分离方法,研究了5种培养基配方对羊肚菌菌丝生长情况(萌发时间、生长速度、菌丝长势)的影响,并结合不同培养基菌丝在显微镜下的表现,筛选出适宜野生羊肚菌生长的最佳培养基,同时通过出菇试验,初步筛选出优良菌株,以期为甘南州食用菌种业自主创新提供坚实的种质基础和种源支撑。结果表明:PDA培养基为羊肚菌菌丝生长的适宜培养基,菌丝生长速度快,菌丝浓密,菌核形成早,在显微镜下菌丝呈树状,有分支。出菇试验结果显示,ZD和LB为优良菌株,可以进一步进行试验。

Taking wild Morchella fruiting bodies collected from within Gannan prefecture(Zhouqu county,Lintan county,Diebu county)as the experimental materials,tissue separation methods were used,the effects of five culture medium formulations on the mycelial growth of Morchella(germination time,growth rate,mycelial growth momentum),and combined the performance of different culture medium mycelium under the microscope were investigated,the best culture medium suitable for the growth of wild Morchella were screenech.At the same time,through mushroom emergence experiments,excellent strains were preliminarily screened,in order to provide a solid germplasm foundation and source support for independent innovation in the edible mushroom industry in Gannan prefecture.The results showed that PDA medium was a suitable medium for the growth of Morchella mycelium,with fast growth rate,dense mycelium,early formation of fungal nuclei,and branching mycelium under a microscope.The results of the mushroom emergence test showed that ZD and LB were excellent strains and could be further tested.