血清miR-142-3p、HMGB1水平与急性胰腺炎患者病情及预后的关系

Relationships between serum miR-142-3p and HMGB1 expression and condition and prognosis in patients with acute pancreatitis

目的探讨急性胰腺炎(AP)患者血清微小核糖核酸-142-3p(miR-142-3p)、高迁移率族蛋白B1(HMGB1)水平与病情及预后的关系。方法选取AP患者253例(AP组),健康体检者100名(对照组),根据病情将AP患者分为轻症组73例、中度重症组99例、重症组81例,根据预后将AP患者分为死亡组25例、存活组228例。分别于AP组入院时、对照组体检时用实时荧光定量聚合酶链反应检测血清miR-142-3p,用酶联免疫吸附法检测血清HMGB1;通过TargetScan数据库预测miR-142-3p与HMGB1的结合位点;Pearson相关法分析AP患者血清miR-142-3p、HMGB1表达的相关性;收集AP患者预后相关资料,多因素Logistic回归分析血清miR-142-3p、HMGB1对预后的影响;受试者工作特征曲线分析血清miR-142-3p、HMGB1水平对AP患者死亡的预测价值。结果与对照组比较,AP组血清miR-142-3p水平低,HMGB1水平高(P均<0.05)。miR-142-3p与HMGB1存在结合位点。AP患者血清miR-142-3p水平与HMGB1水平呈负相关(r=-0.732,P<0.05)。轻症组、中度重症组、重症组血清miR-142-3p水平依次降低(P均<0.05),HMGB1水平依次升高(P均<0.05)。AP患者预后的独立危险因素为重症AP、ICU停留时间长、HMGB1水平升高,独立保护因素为miR-142-3p水平升高(P均<0.05)。血清miR-142-3p联合HMGB1预测AP患者预后的曲线下面积为0.900,大于二者单独预测的曲线下面积(P均<0.05)。结论血清miR-142-3p、HMGB1水平变化与AP患者病情和预后有关,二者联合对AP患者预后的预测价值较高。

Objective To investigate the relationships between the expression of serum microRNA-142-3p(miR-142-3p)and high-mobility group box 1(HMGB1)and the condition and prognosis of acute pancreatitis(AP)patients.Methods Totally 253 AP patients(AP group)and 100 physically examined healthy individuals(control group)were se-lected.AP patients were divided into the mild AP group(73 cases),moderately severe AP group(99 cases),and severe AP group(81 cases)according to the severity of the disease,and they were divided into the death group(25 cases)and the survival group(228 cases)according to the living condition.The serum miR-142-3p was detected by real-time quanti-tative fluorescence PCR and serum HMGB1 was detected by enzyme-linked immunosorbent assay at the time of admission in the AP group and at the time of physical examination in the control group,respectively.The binding sites of miR-142-3p and HMGB1 were predicted by TargetScan database,and Pearson correlation was used to analyze the correlation of se-rum miR-142-3p and HMGB1 expression in AP patients.We collected information about the prognosis of patients with AP.Multifactorial Logistic regression was used to analyze the effects of serum miR-142-3p and HMGB1 on the prognosis;receiver operating characteristic curve was used to analyze the predictive value of serum miR-142-3p and HMGB1 levels on death in AP patients.Results Compared with the control group,serum miR-142-3p expression was reduced and HMGB1 expression was elevated in the AP group(both P<0.05).The miR-142-3p and HMGB1 had a binding site,and their serum expression was negatively correlated in AP patients(r=–0.732,P<0.05).Serum miR-142-3p expression de-creased sequentially in the mild AP group,moderately severe AP group,and severe AP group,and HMGB1 expression in-creased in turn(all P<0.05).The independent risk factors for death in patients with AP were severe AP,prolonged ICU stay,and elevated HMGB1,and the independent protective factor was elevated miR-142-3p(all P<0.05).The area un-der the curve of serum miR-142-3p combined with HMGB1 in predicting death in AP patients was 0.900,which was great-er than that of either alone(both P<0.05).Conclusion The changes of serum miR-142-3p and HMGB1 levels are relat-ed to the condition and prognosis of AP patients,and the detection of serum miR-142-3p combined with HMGB1 is of high value in predicting the prognosis of AP patients.