全血采集去白悬浮红细胞当日与冷藏24h后制备质量比较

Comparison of Preparation Quality Between Whole Blood Collection and White Blood Cell Suspension on the Same Day and After Refrigeration for 24 Hours

目的比较全血采集去白悬浮红细胞当日与冷藏24 h后制备质量的影响。方法选择2023年1—10月青岛市中心血站的245份400 mL全血,纳入观察组,另245份400 mL全血纳入对照组。观察组在4℃冷藏条件下保存24 h后进行去白悬浮红细胞制备,对照组在采集当日进行去白悬浮红细胞制备。比较2组去白悬浮红细胞的滤白时间、滤器损失血量、细胞计数、代谢性指标及游离血红蛋白值、血浆溶血率等质量指标。结果2组滤器损失血量比较,差异无统计学意义(P>0.05);观察组去白悬浮红细胞容量大于对照组,滤白时间长于对照组(P<0.05)。2组血红蛋白值、红细胞数比较,差异无统计学意义(P>0.05),观察组平均红细胞体积为(90.97±4.79)fL,大于对照组的(89.63±5.15)fL(P<0.05)。2组钾离子、钠离子及pH水平和游离血红蛋白含量比较,差异无统计学意义(P>0.05)。观察组血浆溶血率为4.49%(11/245),高于对照组的1.22%(3/245)(P<0.05)。结论与全血采集当日相比,冷藏24h对去白悬浮红细胞制备的滤器损失血量无明显影响,但会增加去白悬浮红细胞容量,延长滤白时间,且对检测的细胞计数、血浆溶血率具有一定的影响。

Objective To compare the effects of whole blood collection day and refrigeration for 24 hours on the quality of preparing white blood cell suspension.Methods A total of 245 samples of 400 mL whole blood from the Qingdao Blood Center from January to October 2023 were selected and included in the observation group.The other 245 samples of 400 mL whole blood were included in the control group.The observation group was stored under 4℃refrigeration conditions for 24 hours before preparing white blood cell suspension.The control group underwent white blood cell suspension preparation on the day of collection.The filtration time,filter blood loss,cell count,metabolic indicators,free hemoglobin value,plasma hemolysis rate,and other quality indicators between two groups of white blood cell suspension were compared.Results There was no statistically significant difference in blood loss between the two groups of filters(P>0.05).The observation group had a larger volume of suspended red blood cells and a longer filtration time than the control group(P<0.05).There was no statistically significant difference in hemoglobin values and red blood cell counts between the two groups(P>0.05).The average red blood cell volume(90.97±4.79)fL in the observation group was greater than that(89.63±5.15)fL in the control group(P<0.05).There was no statistically significant difference in the levels of potassium ions,sodium ions,pH,and free hemoglobin content between the two groups(P>0.05).The plasma hemolysis rate in the observation group was 4.49%(11/245),which was higher than 1.22%(3/245)of the control group(P<0.05).Conclusion Compared with the day of whole blood collection,refrigeration for 24 hours has no significant effect on the loss of blood volume in the filter prepared by removing white suspended red blood cells.However,it increases the capacity of white suspended red blood cells,prolongs the filtration time,and has a certain impact on the cell count and plasma hemolysis rate detected.