S100钙结合蛋白A9与Toll样受体4在糖尿病视网膜病变患者玻璃体液的表达及临床意义

Expression and significance of S100A9 and TLR4 in vitreous of patients with diabetic retinopathy

目的了解糖尿病视网膜病变患者玻璃体液中S100钙结合蛋白A9(S100A9)及Toll样受体4(TLR4)的表达并讨论其临床意义。方法选取2022年8月至2023年8月于合肥市第二人民医院行手术治疗的患者40例。研究组为糖尿病视网膜病变需行玻璃体切割手术治疗的20例患者,对照组为特发性黄斑裂孔或黄斑前膜需行玻璃体切除手术的20例患者。收集患者玻璃体液,用ELISA法测定其S100A9及TLR4蛋白浓度。结果研究组玻璃体液S100A9浓度为(5.95±1.55)μg/L高于对照组(2.19±0.76)μg/L,差异有统计学意义(P<0.05);研究组玻璃体液TLR4浓度为(1.54±0.79)μg/L,高于对照组(0.34±0.18)μg/L,差异有统计学意义(P<0.05);研究组玻璃体液S100A9与TLR4呈正相关(r=0.72,P<0.05)。结论糖尿病视网膜病变患者玻璃体液中S100A9及TLR4水平增高,提示炎症参与其发病过程。

Objective To investigate the expression and significance of S100A9 and TLR4 in vitreous fluid of patients with diabetic retinopathy.Methods A total of 40 patients who underwent vitrectomy at the Second People′s Hospital of Hefei from August 2022 to August 2023 were selected.Among them,20 patients with diabetic retinopathy were included as the experimental group,and 20 patients with macular hole or epiretinal membrane were included as the control group.Vitreous fluid samples were collected,and the protein concentrations of S100A9 and TLR4 were measured using the ELISA method.Results The concentration of S100A9 in the experimental group was(5.95±1.55)μg/L,which was significantly higher than that in the control group(2.19±0.76)μg/L,and the difference was statistically significant.The TLR4 concentration in the experimental group was(1.54±0.79)μg/L significantly higher than that in the control group of(0.34±0.18)μg/L,and the difference was statistically significant(P<0.05).There was a positive correlation between S100A9 and TLR4 in the experimental group(r=0.72,P<0.05).Conclusions The increased levels of S100A9 and TLR4 in vitreous fluids of diabetic retinopathy suggest that inflammation may be involved in the process of its elevelopment.