基于免疫组化SP法检测AEG-1在不同分化程度和分期的卵巢癌患者中的阳性表达及病理特点

Detection of the positive expression and pathological characteristics of astrocyte up-regulated gene-1 in ovarian cancer patients with different differentiation degrees and stages based on ininiunohistocheniical streptavidin-perosidase method

目的探讨基于免疫组化链霉菌抗生物素蛋白-过氧化物酶连结(SP)法检测星形胶质细胞上调基因-1(AEG-1)在不同分化程度和分期的卵巢癌患者中的阳性表达及病理特点。方法选取2018年10月至2023年1月哈尔滨医科大学附属第三医院收治的97例卵巢癌患者作为研究对象,所有患者均行手术切除治疗,并经过病理检查确诊。采用免疫组化SP法检测卵巢病变组织肿瘤中的AEG-1表达情况,分析AEG-1阳性表达情况、染色强度评分、病理特点。结果染色强度评分为(3.14±0.76)分。卵巢癌组织中AEG-1阳性表达率为83.51%,AEG-1阳性信号未出现在肿瘤周边正常卵巢组织。黏液性卵巢癌和卵巢浆液性囊腺癌患者卵巢癌组织中AEG-1阳性表达率比较,差异无统计学意义(P>0.05)。卵巢癌患者国际妇产科联盟(FIGO)各分期、淋巴结有无转移的阳性表达比较,差异具有统计学意义(P<0.05);卵巢癌患者年龄、各分化程度阳性表达率比较,差异无统计学意义(P>0.05)。结论AEG-1会促进卵巢癌的发生、发展,其可用于判断卵巢癌的恶性程度、疾病进展情况。

Objective To explore the positive rate expression and pathological characteristics of astrocyte up-regulated gene-1(AEG-1)in different differentiation degrees and stages of ovarian cancer based on immunohistochemical streptavidin-peroxidase(SP)method.Methods A total of 97 patients with ovarian cancer admitted to the Third Affiliated Hospital of Harbin Medical University from October 2018 to January 2023 were selected as the study subjects.All patients underwent surgical resection and were diagnosed by pathological examination.The expression of AEG-1 in ovarian lesions was detected by immunohistochemical SP method.The positive expression of AEG-1,staining intensity score and pathological features were analyzed.Results The staining intensity score was 3.14±0.76 points.The positive rate of AEG-1 expression in ovarian cancer tissues was 83.51%,and the positive signal of AEG-1 did not appear in normal ovarian tissues around the tumor.There was no significant difference in the positive rate of AEG-1 expression in ovarian cancer tissues between patients with mucinous ovarian cancer and ovarian serous cystadenocarcinoma(P>0.05).There were statistically significant differences in the positive expression rates of international federation of gynecology and obstetrics(FIGO)staging and lymph node metastasis in patients with ovarian cancer(P<0.05).There was no significant difference in the positive expression rate of ages and differentiation degrees of ovarian cancer patients(P>0.05).Conclusions AEG-1 can promote the occurrence and development of ovarian cancer,which can be used to judge the malignant degree and disease progression of ovarian cancer.