薰衣草精油调节Ⅰ型代谢型谷氨酸受体对产前应激子代大鼠抑郁样行为的作用研究

Effect of Lavender Essential Oil on Modulating Type I Metabolic Glutamate Receptor on Depression-like Behavior in Prenatal Stressed Offspring Rats

为探讨薰衣草精油对产前应激子代大鼠抑郁恢复的影响及潜在机制,采用SD大鼠构建产前束缚应激模型,产后子代雄鼠分为对照组(CON组)、产前应激组(PS组)、薰衣草精油组(PS-LEO组)和生理盐水组(PS-Saline组),通过蔗糖偏好实验和强迫游泳实验评估子代大鼠抑郁样行为的恢复情况。采用酶联免疫吸附试验(ELISA)和实时荧光定量PCR实验,评估Ⅰ型代谢型谷氨酸相关受体mGluR1、mGluR5和EAAT2的蛋白水平和mRNA水平变化情况。蔗糖偏好实验和强迫游泳实验结果显示,薰衣草精油改善产前应激子鼠抑郁样行为,ELISA和PCR结果显示,薰衣草精油干预后mGluR1和mGluR5蛋白水平显著降低,使得海马区EAAT2蛋白水平显著升高(P<0.05),说明薰衣草精油可以通过调节mGluR1、mGluR5和EAAT2的水平来改善产前应激子鼠抑郁样行为。

In order to investigate the effects of lavender essential oil on depression recovery of prenatal stressed offspring rats and its potential mechanism,the study establishes a prenatal restraint stress model with SD rats;divides postpartum male offspring rats into control group(CON group),prenatal stress group(PS group),lavender essential oil group(PS-LEO group)and normal saline group;and evaluates the recovery of depression-like behavior in offspring rats with sucrose preference test and forced swimming test.The study evaluates the changes in protein levels and mRNA levels of glutamate-related receptors mGluR1,mGluR5 and EAAT2 with Enzyme-linked immunosorbent assay(ELISA)and real-time fluorescence quantitative PCR.The results of sucrose preference experiment and forced swimming experiment show that lavender oil improved depression-like behavior in prenatal stressed rats.ELISA and PCR results show that mGluR1 and mGluR5 protein levels are significantly decreased after lavender essential oil intervention,and EAAT2 protein levels in hippocampus are significantly increased(P<0.05).This indicates that lavender essential oil can improve the depression-like behavior of prenatal stressed rats by regulating the levels of mGluR1,mGluR5 and EAAT2.