没食子-甘草凝胶对实验性牙周炎模型局部炎症及Th17/Tregs的影响

Gallnut-glycyrrhizae gel on local inflammation and Th17/Tregs balance in experimental periodontitis model

目的 分析没食子-甘草凝胶调节牙周炎局部Th17/Tregs的影响,阐明其在牙周免疫调节及牙周炎治疗中的意义。方法 将SD大鼠随机等量分为对照组、牙周炎组及观察组,采用结扎法建立病理模型。观察组给予自制没食子甘草凝胶进行干预。评价各组实验动物牙龈出血、牙齿松动以及牙槽骨吸收情况。分析各组牙龈及外周血中Th17/Tregs的比例差异及IL-17、IL-10以及TGF-β的表达水平。结果 与对照组比较,牙周炎组牙龈出血、牙齿松动及牙槽骨吸收水平均升高,观察组仅牙齿松动升高(P<0.05)。牙周炎组与对照组比较,牙龈中Th17比例升高、Tregs下降(P<0.05),观察组未表现出此现象。牙周炎组与对照组比较IL-17升高,而IL-10、TGF-β下降(P<0.05),而观察组仅IL-10以及TGF-β的mRNA低于对照组(P<0.05),余差异无统计学意义(P>0.05)。结论 没食子-甘草凝胶有效缓解牙龈出血、牙齿松动、牙槽骨吸收及牙龈Th17比例异常,对牙周炎有治疗效果。

Objective To analyze the effect of gallnut-glycyrrhizae gel on regulating the local Th17/Tregs balance in periodontitis and elucidate its significance in periodontal immune regulation and periodontitis treatment.Methods SD rats were randomly divided into a control group,a periodontitis group,and an observation group in equal proportions.A pathological model was established using the ligation method.The observation group was intervened with self-made gallnut-glycyrrhizae gel.The gingival bleeding,tooth mobility,and alveolar bone resorption were evaluated in each group.The differences in the ratio of Th17/Tregs and the expression levels of IL-17,IL-10,and TGF-βin gingival tissue and peripheral blood were analyzed.Results Compared with the control group,the periodontitis group showed increased levels of gingival bleeding,tooth mobility,and alveolar bone resorption,while only tooth mobility increased in the observation group(P<0.05).Compared with the control group,the periodontitis group exhibited an increase in the proportion of Th17 and a decrease in Tregs in gingival tissue(P<0.05),which was not observed in the observation group.The periodontitis group had elevated IL-17 and decreased IL-10 and TGF-βlevels compared to the control group(P<0.05),while the observation group only showed lower mRNA levels of IL-10 and TGF-βcompared to the control group(P<0.05),with no significant differences in the other indicators(P>0.05).Conclusion Gallnut-glycyrrhizae gel effectively alleviates gingival bleeding,tooth mobility,alveolar bone resorption,and abnormal Th17 proportion in gingival tissue,demonstrating a therapeutic effect on periodontitis.