“柔筋温通”手法干预家兔骨骼肌慢性损伤的作用机制

The Mechanism of"Softening Tendons and Warming Tong"Manipulation to Intervene Chronic Skeletal Muscle Injury in Rabbits

目的〓探讨“柔筋温通”手法干预家兔骨骼肌慢性损伤的作用机制。方法选用40只健康成年家兔,30只造模成功后分别设为实验组、对照组、模型组各10只,余下10只设为正常组。实验组采用“柔筋温通”手法干预,对照组采用地塞米松药物治疗,共治疗10次,模型组和正常组均正常喂养,治疗结束后取材检测,采用ELISA法检测TNF-α、IL-6的含量以及采用Western blot检测TGF-β1、CTGF的蛋白表达。结果实验组兔骨骼肌中TNF-α、IL-6含量显著降低;CTGF、TGF-β1蛋白表达明显降低。结论通过研究,“柔筋温通”手法通过降低骨骼肌损伤局部炎性因子(TNF-α、IL-6)的含量以及通过降低的TGF-β1、CTGF的蛋白表达,来抑制炎性粘连和炎症纤维组织增生,促进骨骼肌慢性损伤的修复。

Objective To explore the mechanism of"softening tendons and warming Tong"manipulation to intervene chronic skeletal muscle injury in rabbits.Methods 40 healthy adult rabbits were selected,30 rabbits were set as experimental group,control group and model group,and the remaining 10 rabbits were set as normal group.The experimental group used"soft tendons warming Tong"manipulation intervention,the control group used dexamethasone drug treatment,co-treatment 10 times,the model group and the normal group were fed normally.After treatment,samples were collected for detection,and TNF-αand IL-6 were detected by ELISA,and the protein expression of TGF-β1 and CTGF were detected by Western blot.Results The contents of TNF-αand IL-6 in skeletal muscle were significantly decreased.The protein expressions of CTGF and TGF-β1 were significantly decreased.Conclusion Through the study,"softening tendons and warming Tong"manipulation can reduce the content of local inflammatory factors(TNF-α,IL-6)in skeletal muscle injury as well as reduce the content of inflammatory factors TGF-β1,CTGF protein expression,to inhibit inflammatory adhesion and inflammatory fibrous tissue hyperplasia,promote skeletal muscle chronic damage repair of injury.