液相色谱法测定0.16%噻苯隆·14-羟基芸苔素甾醇可溶液剂质量分数

Analysis of 0.16%Thidiazuron·14-hydroxylated Brassinosteroid SL by HPLC

本文采用液相色谱法测定0.16%噻苯隆·14-羟基芸苔素甾醇可溶液剂中噻苯隆和14-芸苔素甾醇的质量分数,使用C18不锈钢反相色谱柱和紫外可变波长检测器,分别以甲醇和水及乙腈和水为流动相,用外标法对有效成分进行定量分析。结果表明:噻苯隆和14-芸苔素甾醇内酯相关系数为0.9996和0.9994,标准偏差分别为:0.0013%、0.0005%;相对标准偏差分别为:0.86%、5.17%;平均回收率分别为:99.76%、98.80%。

A method was developed for the determination of 0.16%Thidiazuron·14-hydroxylated brassinosteroid SL by high-performance liquid chromatography on a C18 column with a variable wave length UV detector.The mobile phase consisted of methanol and water,acetonitrile and water.The results showed that linear correlations of 0.9996 and 0.9994,standard deviations of 0.0013%and 0.0005%,coefficients of variation of 0.86%and 5.17%,and average recoveries of 99.76%and 98.80%for Thidiazuron and14-hydroxylated brassinosteroid,respectively.In Conclusion,the method is sensitive and quick,making it suitable for the analysis of 0.16%Thidiazuron·14-hydroxylated brassinosteroid SL.