鸡源鸭疫里默氏杆菌的分离鉴定与生物学特性研究

Isolation,Identification and Biological Characterization of Riemerella anatipestifer from Chicken

为探究山东、安徽、河北、宁夏等地区育成阶段蛋鸡群出现的一种以输卵管干酪物为主要病理特征的疾病病因,该研究对临床病理样本进行细菌分离培养,并对分离菌株进行形态学、分子生物学、血清学、动物回归试验和药敏试验等方面的研究与分析。结果显示,13株分离菌株均为鸭疫里默氏杆菌,以血清型7型为主,其OmpA基因已进化为2大分支,动物回归试验可复制出相同临床症状,药敏试验显示分离菌株对氟苯尼考、多西环素、大观霉素、头孢类药物较为敏感。该研究结果为鸭疫里默氏杆菌从不同易感宿主上的分离鉴定及菌株的遗传进化分析提供了理论基础,对家禽感染鸭疫里默氏杆菌的预防与控制提供了参考依据。

To investigate the cause of caseous exudate in oviducts of growing layers in parts of Shandong,Anhui,Hebei,Ningxia and other regions,bacteria were isolated and cultured from the clinical pathological samples.Morphological,molecular biology,serological,animal regression and drug sensitivity tests for these isolated bacteria were carried out.The results showed that the 13 isolated bacteria were Riemerella anatipestifer,mainly serotype 7,and the OmpA genes had evolved into two branches.The animal regression test could replicate the same clinical symptoms,and the drug sensitivity test showed that the isolated bacteria were more sensitive to florfenicol,doxycycline,spectinomycin and cephalosporins.This study provides a theoretical basis for the isolation and identification of Riemerella anatipestifer from different susceptible hosts and the genetic evolution analysis of strains,and also provides reference for the prevention and control of Riemerella anatipestifer infection in poultry.