适用于电针及灌胃干预的大鼠动眼神经损伤模型

Rat Model of Oculomotor Nerve Injury Which is Suitable for Electroacupuncture and Gavage

目的本研究复制现有大鼠颅内动眼神经损伤模型,并提出了一种新的眶腔内钳夹动眼神经的方法,评价两种模型各自的优势。方法以雄性SD大鼠为实验动物,对2种不同动眼神经损伤模型制备方法进行术后不同干预对照研究。蝶腭孔入路组:自眼眶上缘开口,钳夹动眼神经入眶起始段10 s。颅底入路组:咬除颞骨,钳夹动眼神经幕下段10 s。以模型大鼠成功率、存活率、生存状态为观察指标,评价大鼠模型对灌胃及眶内电针干预的适用性。结果成功率:造模24 h后蝶腭孔入路组成功12只,成功率92.31%(12/13);颅底入路组成功12只,成功率34.29%(12/35)。存活率:灌胃7天后:蝶腭孔入路组存活6只,存活率100.00%(6/6);颅底入路组存活4只,存活率66.67%(4/6)。眶内电针7天后:蝶腭孔入路组存活5只,存活率83.33%(5/6);颅底入路组存活3只,存活率50.00%(3/6)。大鼠生存状态(0-20分,分值越小状态越好):造模24 h后蝶腭孔入路组状态评分4.11±0.90,颅底入路组状态评分12.39±1.378;灌胃7天后蝶腭孔入路组状态评分2.50±1.05,颅底入路组状态评分14.50±0.58。眶内电针干预7天后蝶腭孔入路组状态评分2.00±1.22,颅底入路组状态评分15.33±0.58。造模24 h后蝶腭孔入路组模型鼠成功率、存活率和大鼠生存状态均优于颅底入路组(P<0.05);灌胃及眶内电针干预7天后蝶腭孔入路组大鼠存活率和生存状态优于颅底入路组大鼠(P<0.05)。结论经蝶腭孔入路制备的动眼神经损伤模型鼠更适用于灌胃及眶内电针干预后动眼神经损伤后神经修复基础研究,颅底入路制备的动眼神经损伤模型鼠更适用于动眼神经颅内段损伤后的基础研究。

Objective To duplicate the existing model of intracranial oculomotor nerve injury in rats,and propose a new method of orbital cavity clamping oculomotor nerve,evaluate the respective advantages of the two models.Methods Male SD rats were used as experimental animals,and two different methods of preparing oculomotor nerve injury models were compared.Sphenopalatine foramen approach group:clamp the initial segment of the oculomotor nerve from the opening of the upper edge of the orbit for 10 s.The skull base approach group,the temporal bone was removed and the infratentorial segment of the oculomotor nerve was clampped for 10 s.Taking the success rate,survival rate and survival status of the model rats as observation indicators,the applicability of the model rats to the intervention of gastric perfusion was and intraorbital electroacupuncture evaluated.Results The success rate after molding 24 h:In the sphenopalatine foramen approach group was 92.31%(12/13);In the skull base approach group was 34.29%(12/35).Survival rate:7 d after gavage:In the sphenopalatine foramen approach group,6 patients survived,with a survival rate of 100.00%(6/6).Four patients survived in the skull base approach group,with a survival rate of 66.67%(4/6).7 days after orbital electroacupuncture:Five patients survived in the sphenopalatine foramen approach group(83.33%,5/6).Three patients survived in the skull base approach group,with a survival rate of 50.00%(3/6).Survival state of rats(0-20 points,the smaller the score,the better):24 h after modeling,the status score of sphenopalatine foramina approach group was 4.11±0.90,and that of skull base approach group was 12.39±1.378.After 7 days of intragastric administration,the score of sphenopalatine foramina approach group was 2.50±1.05,and the score of skull base approach group was 14.50±0.58.The score of sphenopalatine foramina approach group was 2.00±1.22 and the score of skull base approach group was 15.33±0.58 after 7 days of intraorbital electroacupuncture intervention.The success rate,survival rate and survival state of rats in sphenopalatine foramina approach group were better than those in skull base approach group 24 h after modeling(P<0.05);The survival rate and survival state of rats in sphenopalatine foramina approach group were better than those in skull base approach group 7 days after gavage and orbital electroacupuncture intervention(P<0.05).Conclusion The model of oculomotor nerve injury prepared by sphenopalatine foramina approach is more suitable for the basic research of nerve repair after the intervention of gastric gavage and orbital electroacupuncture.The model rats with oculomotor nerve injury prepared by the base approach are more suitable for the basic research after the injury of the intracranial segment of the oculomotor nerve.