三七根茎对缺血再灌注诱发的急性肾损伤的保护作用

Protective effect of Panax notoginseng rhizomes on acute kidney injury induced by ischemia-reperfusion

目的 探究三七根茎对肾缺血再灌注损伤(RIRI)的保护作用。方法 将雄性SD大鼠随机分为假手术组、模型组、三七根茎组,预处理给药7 d后建立双侧RIRI模型,并于再灌注24 h后收集大鼠血液、双肾组织。利用血液样本进行血肌酐(SCr)、尿素氮(BUN)、单核细胞数(MONO)和中性粒细胞数(NEUT)含量测定,评估肾脏功能。利用肾脏组织进行丙二醛(MDA)含量测定,检测肾脏组织氧化应激情况;进行HE染色,观察肾脏组织病理性改变;进行CD86免疫荧光染色,评价肾脏组织促炎巨噬细胞侵润情况。结果 与假手术组相比,模型组SCr、BUN、MONO、NEUT和MDA含量增加(P<0.001);肾小管形态病理性改变,损伤评分增加(P<0.001);促炎巨噬细胞侵润严重,CD86阳性细胞数增加(P<0.001)。与模型组相比,三七根茎组显著降低了SCr、BUN、MONO、NEUT和MDA含量(P<0.01);降低了肾小管形态病理损伤和评分(P<0.001);减少了促炎巨噬细胞侵润情况和CD86阳性细胞数(P<0.001)。结论 三七根茎能通过抗炎、抗氧化来保护肾脏组织,减少缺血再灌注损伤,并抑制CD86促炎巨噬细胞侵润。

Objective To investigate the protective effect of Panax notoginseng rhizomes on renal ischemia-reperfusion injury(RIRI).Methods Male SD rats were randomly divided into a sham group,a model group,and a Panax notoginseng rhizomes group,and a bilateral RIRI model was established after pretreatment administration for 7 days,and blood and both kidneys of rats were collected after 24 hours of reperfusion.Blood samples were utilized for the determination of serum creatinine(SCr),blood urea nitrogen(BUN),monocyte(MONO)and neutrophil(NEUT)levels to assess renal function.Kidney tissue was used for the determination of malondialdehyde(MDA)content to detect oxidative stress;HE staining was carried out to observe the pathologic changes in renal tissues;and immunofluorescence staining with CD86 was performed to evaluate the pro-inflammatory macro-phage infiltration of renal tissues.Results Compared with the sham group,the model group had increased levels of SCr,BUN,MONO,NEUT,and MDA(P<O.001),increased pathologic changes in renal tubular morphology and injury scores(P<0.001),and increased severity of pro-inflammatory macrophage infiltration and number of CD86-positive cells(P<0.001).Compared with the model group,the Panax notoginseng rhizomes group significantly reduced SCr,BUN,MONO,NEUT,and MDA levels(P<0.01),reduced renal tubular morphopathologic damage and scores(P<0.001),and reduced pro-inflammatory macrophage infiltration and CD86-positive cell count(P<0.001).Conclusion Panax notoginseng rhizomes can protect kidney tissue by anti-inflammation and anti-oxidation,reduce ischemia-reperfusion injury,and inhibit the infiltration of CD86 pro-inflammatory macrophages.